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Piericiden A Sensitivity, Site 1 Phosphorylation, And Reduced Nicotinamide Adenine Dinucleotide Dehydrogenase During Iron-Limited Growth Of Candida Utilis, John G. Cobley, T. P. Singer, H. Beinert, S. Grossman

Chemistry Faculty Publications

It has been reported that cells of Candida utilis, grown in continuous culture under iron-limited conditions, develop site 1 phosphorylation, without the appearance of piericidin sensitivity and without changes in the iron-sulfur centers of NADH dehydrogenase, on aeration in the presence of cycloheximide, as well as on increasing the supply of iron during growth. These findings were reinvestigated in the present study. The parameters and properties followed during these transitions were sensitivity of NADH oxidation to piericidin, presence or absence of coupling site 1, EPR signals appearing on reduction with NADH or dithionite, the specific activities of NADH oxidase, NADH-ferricyanide …