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Physical Sciences and Mathematics Commons

Open Access. Powered by Scholars. Published by Universities.®

Chemistry

Rowan University

Series

2019

Leakage assay

Articles 1 - 1 of 1

Full-Text Articles in Physical Sciences and Mathematics

Correlating Lipid Membrane Permeabilities Of Imidazolium Ionic Liquids With Their Cytotoxicities On Yeast, Bacterial, And Mammalian Cells, Kendall Cook, Katharine Tarnawsky, Alana J. Swinton, Daniel D. Yang, Alexandria S. Senetra, Gregory A. Caputo, Benjamin Carone, Timothy Vaden Jun 2019

Correlating Lipid Membrane Permeabilities Of Imidazolium Ionic Liquids With Their Cytotoxicities On Yeast, Bacterial, And Mammalian Cells, Kendall Cook, Katharine Tarnawsky, Alana J. Swinton, Daniel D. Yang, Alexandria S. Senetra, Gregory A. Caputo, Benjamin Carone, Timothy Vaden

Faculty Scholarship for the College of Science & Mathematics

Alkyl-imidazolium chloride ionic liquids (ILs) have been broadly studied for biochemical and biomedical technologies. They can permeabilize lipid bilayer membranes and have cytotoxic effects, which makes them targets for drug delivery biomaterials. We assessed the lipid-membrane permeabilities of ILs with increasing alkyl chain lengths from ethyl to octyl groups on large unilamellar vesicles using a trapped-fluorophore fluorescence lifetime-based leakage experiment. Only the most hydrophobic IL, with the octyl chain, permeabilizes vesicles, and the concentration required for permeabilization corresponds to its critical micelle concentration. To correlate the model vesicle studies with biological cells, we quantified the IL permeabilities and cytotoxicities on …