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Full-Text Articles in Physical Sciences and Mathematics
Aptamer-Based Assay For Detection Of Ochratoxin A, Amanda Nicole Bartley
Aptamer-Based Assay For Detection Of Ochratoxin A, Amanda Nicole Bartley
FIU Electronic Theses and Dissertations
Ochratoxin A (OTA) is a potent mycotoxin found in a wide range of agricultural products that has been linked to mitochondrial damage and renal disease. The standard methods for OTA analysis currently rely on the use of high-performance liquid chromatography (HPLC) coupled to fluorescence detection or mass spectrometry. Toward a highthroughput analysis of OTA, a single-stranded DNA aptamer, modified with a fluorophore, coupled to a complementary sequence, modified with a FRET-based quencher that dissociates in the presence of the target toxin, is proposed. In order to integrate “target trapping,” aptamer immobilization methods were explored to mediate interference issues. Assays were …
Molecular Recognition Of Methionine-Terminated Peptides By Cucurbit[8]Uril, Zoheb Hirani, Hailey F. Taylor, E. F. Babcock, Andrew T. Bockus, C. D. Varnado Jr., Christopher W. Bielawski, Adam R. Urbach
Molecular Recognition Of Methionine-Terminated Peptides By Cucurbit[8]Uril, Zoheb Hirani, Hailey F. Taylor, E. F. Babcock, Andrew T. Bockus, C. D. Varnado Jr., Christopher W. Bielawski, Adam R. Urbach
Chemistry Faculty Research
This Article describes the molecular recognition of peptides containing an N-terminal methionine (Met) by the synthetic receptor cucurbit[8]-uril (Q8) in aqueous solution and with submicromolar affinity. Prior work established that Q8 binds with high affinity to peptides containing aromatic amino acids, either by simultaneous binding of two aromatic residues, one from each of two different peptides, or by simultaneous binding of an aromatic residue and its immediate neighbor on the same peptide. The additional binding interface of two neighboring residues suggested the possibility of targeting nonaromatic peptides, which have thus far bound only weakly to synthetic receptors. A peptide library …
Effect Of Loading Method On A Peptide Substrate Reporter In Intact Cells [Post-Print], Rahuljeet Chadha, Grigorii Kalminskii, Allison Tierney, Joshua Knopf, Lorena Lazo De La Vega, Berjana Mcelrath, Michelle Kovarik
Effect Of Loading Method On A Peptide Substrate Reporter In Intact Cells [Post-Print], Rahuljeet Chadha, Grigorii Kalminskii, Allison Tierney, Joshua Knopf, Lorena Lazo De La Vega, Berjana Mcelrath, Michelle Kovarik
Faculty Scholarship
Studies of live cells often require loading of exogenous molecules through the cell membrane; however, effects of loading method on experimental results are poorly understood. Therefore, in this work, we compared three methods for loading a fluorescently labeled peptide into cells of the model organism Dictyostelium discoideum. We optimized loading by pinocytosis, electroporation, and myristoylation to maximize cell viability and characterized loading efficiency, localization, and uniformity. We also determined how the loading method affected measurements of enzyme activity on the peptide substrate reporter using capillary electrophoresis. Loading method had a strong effect on the stability and phosphorylation of the …
Synthesis, Optical Spectroscopy And Laser Potential Of Pyrylium Tosylates, Jung Jae Koh, Christina Inbok Lee, Mihaela Alexa Ciulei, Haesook Han, Pradip Kumar Bhowmik, Vladimir Kartazaev, Swapan Kumar Gayen
Synthesis, Optical Spectroscopy And Laser Potential Of Pyrylium Tosylates, Jung Jae Koh, Christina Inbok Lee, Mihaela Alexa Ciulei, Haesook Han, Pradip Kumar Bhowmik, Vladimir Kartazaev, Swapan Kumar Gayen
Chemistry and Biochemistry Faculty Research
Safe and inexpensive methods for synthesis of a series of four substituted 2,4,6-triphenylyrylium tosylate salts with different substituents are reported. The synthesis methods use p-toluenesulfonic acid monohydrate instead of conventional acid catalysts including perchloric acid or boron trifluoride diethyl etherate that pose explosion danger and difficult storage problems, respectively. The chemical structures of these salts were established using FTIR, 1H and 13C NMR spectroscopic techniques and elemental analysis. Thermogravimetric analysis (TGA) showed that these salts have good thermal stability, and differential scanning calorimetry (DSC) analysis showed that they have lower melting transitions than the corresponding tetrafluoroborate and perchlorate salts. Solutions …