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Full-Text Articles in Physical Sciences and Mathematics
The Association Of Nadph With The Guanine Nucleotide Exchange Factor From Rabbit Reticulocytes: A Role Of Pyridine Dinucleotides In Eukaryotic Polypeptide Chain Initiation, Jaydev N. Dholakia, Timothy C. Muesser, Charles L. Woodley, Lawrence J. Parkhurst, Albert J. Wahba
The Association Of Nadph With The Guanine Nucleotide Exchange Factor From Rabbit Reticulocytes: A Role Of Pyridine Dinucleotides In Eukaryotic Polypeptide Chain Initiation, Jaydev N. Dholakia, Timothy C. Muesser, Charles L. Woodley, Lawrence J. Parkhurst, Albert J. Wahba
Chemistry Department: Faculty Publications
The guanine nucleotide exchange factor (GEF) was purified to apparent homogeneity from postribosomal supernatants of rabbit reticulocytes by chromatography on DEAE-celiulose and phosphocellulose, fractionation by glycerol gradients, and chromatography on Mono S and Mono Q (Pharmacia). At the Mono S step GEF is isolated as a complex with the eukaryotic polypeptide chain initiation factor 2 (eIF-2) and is separated from this factor by column chromatography on Mono Q. An emission spectrum characteristic of a reduced pyridine dinucleotide was observed when GEF was subjected to fluorescence analysis. By both coupled enzymatic analysis and chromatography on reverse-phase or Mono Q columns, the …