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Laser Ablation Sample Transfer For Mass Spectrometry, Sung-Gun Park
Laser Ablation Sample Transfer For Mass Spectrometry, Sung-Gun Park
LSU Doctoral Dissertations
In this research, a new ambient sampling technique for mass spectrometry was developed that uses an infrared laser to ablate materials under ambient conditions that are captured in a solvent or on a surface. An infrared optical parametric oscillator (OPO) laser system at 3 μm wavelength was focused onto samples for ablation at atmospheric pressure. The ablated materials were transferred to a solvent or surface. For off-line electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) analysis, the ablated material was captured in a static solvent droplet that was deposited on a MALDI target or flow-injected into a nanoelectrospray source. The …
Analysis Of Protein Aggregates And Synthetic Nanoparticles Using Capillary Electrophoresis With Light Scattering And Fluorescence Detection, Suresh Chandra Regmi
Analysis Of Protein Aggregates And Synthetic Nanoparticles Using Capillary Electrophoresis With Light Scattering And Fluorescence Detection, Suresh Chandra Regmi
LSU Doctoral Dissertations
The goal of the research presented in this dissertation is to develop capillary electrophoresis (CE) based analysis techniques for the characterization of individual particles including amyloid beta (Aâ) peptide aggregates and to apply these methods to study the aggregation of Aâ peptides. Protein self-assembly into ordered aggregates is a common and important process in normal biological function, but this process is also involved in several diseases. Amyloid beta peptide aggregation is linked to Alzheimer’s disease. Thioflavin T (ThT) has been used as a fluorescent probe for the detection of Aâ aggregation. Based on previous observations of false positive fluorescent signals …