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Full-Text Articles in Physical Sciences and Mathematics
Collaborative Learning Exercises For Teaching Protein Mass Spectrometry [Post-Print], Michelle Kovarik, Jill K. Robinson
Collaborative Learning Exercises For Teaching Protein Mass Spectrometry [Post-Print], Michelle Kovarik, Jill K. Robinson
Faculty Scholarship
A collaborative learning module for teaching protein mass spectrometry has been developed to overcome common obstacles to incorporating the modern topic of biological mass spectrometry into the undergraduate chemistry curriculum. Protein mass spectrometry data is provided to eliminate the need for expensive instrumentation, and an instructor’s manual gives necessary details for those unfamiliar with the topic. The first section provides background information on proteins and the field of proteomics. The second section describes the use of electrospray ionization to determine the molecular weight of a protein. The third section shows how to identify a protein using peptide mass mapping, and …
Effect Of Loading Method On A Peptide Substrate Reporter In Intact Cells [Post-Print], Rahuljeet Chadha, Grigorii Kalminskii, Allison Tierney, Joshua Knopf, Lorena Lazo De La Vega, Berjana Mcelrath, Michelle Kovarik
Effect Of Loading Method On A Peptide Substrate Reporter In Intact Cells [Post-Print], Rahuljeet Chadha, Grigorii Kalminskii, Allison Tierney, Joshua Knopf, Lorena Lazo De La Vega, Berjana Mcelrath, Michelle Kovarik
Faculty Scholarship
Studies of live cells often require loading of exogenous molecules through the cell membrane; however, effects of loading method on experimental results are poorly understood. Therefore, in this work, we compared three methods for loading a fluorescently labeled peptide into cells of the model organism Dictyostelium discoideum. We optimized loading by pinocytosis, electroporation, and myristoylation to maximize cell viability and characterized loading efficiency, localization, and uniformity. We also determined how the loading method affected measurements of enzyme activity on the peptide substrate reporter using capillary electrophoresis. Loading method had a strong effect on the stability and phosphorylation of the …