Physical Sciences and Mathematics Commons^™

Interspecies Comparison Of Peptide Substrate Reporter Metabolism Using Compartment-Based Modeling [Post-Print], Allison Tierney, Nhat Pham, Kunwei Yang, Brooks Emerick, Michelle Kovarik

Faculty Scholarship

Peptide substrate reporters are fluorescently labeled peptides that can be acted upon by one or more enzymes of interest. Peptide substrates are readily synthesized and more easily separated than full-length protein substrates; however, they are often more rapidly degraded by peptidases. As a result, peptide reporters must be made resistant to proteolysis in order to study enzymes in intact cells and lysates. This is typically achieved by optimizing the reporter sequence in a single cell type or model organism, but studies of reporter stability in a variety of organisms are needed to establish the robustness and broader utility of these …