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Physical Sciences and Mathematics Commons™
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Full-Text Articles in Physical Sciences and Mathematics
Effect Of Loading Method On A Peptide Substrate Reporter In Intact Cells [Post-Print], Rahuljeet Chadha, Grigorii Kalminskii, Allison Tierney, Joshua Knopf, Lorena Lazo De La Vega, Berjana Mcelrath, Michelle Kovarik
Effect Of Loading Method On A Peptide Substrate Reporter In Intact Cells [Post-Print], Rahuljeet Chadha, Grigorii Kalminskii, Allison Tierney, Joshua Knopf, Lorena Lazo De La Vega, Berjana Mcelrath, Michelle Kovarik
Faculty Scholarship
Studies of live cells often require loading of exogenous molecules through the cell membrane; however, effects of loading method on experimental results are poorly understood. Therefore, in this work, we compared three methods for loading a fluorescently labeled peptide into cells of the model organism Dictyostelium discoideum. We optimized loading by pinocytosis, electroporation, and myristoylation to maximize cell viability and characterized loading efficiency, localization, and uniformity. We also determined how the loading method affected measurements of enzyme activity on the peptide substrate reporter using capillary electrophoresis. Loading method had a strong effect on the stability and phosphorylation of the …
Pseudoelasticity At Large Strains In Au Nanocrystals [Post-Print], X. Wendy Gu, Lindsey A. Hanson, Carissa N. Eisler, Matthew A. Koc, A. Paul Alivisatos
Pseudoelasticity At Large Strains In Au Nanocrystals [Post-Print], X. Wendy Gu, Lindsey A. Hanson, Carissa N. Eisler, Matthew A. Koc, A. Paul Alivisatos
Faculty Scholarship
© 2018 American Physical Society. Pseudoelasticity in metals is typically associated with phase transformations (e.g., shape memory alloys) but has recently been observed in sub-10 nm Ag nanocrystals that rapidly recovered their original shape after deformation to large strains. The discovery of pseudoelasticity in nanoscale metals dramatically changes the current understanding of the properties of solids at the smallest length scales, and the motion of atoms at surfaces. Yet, it remains unclear whether pseudoelasticity exists in different metals and nanocrystal sizes. The challenge of observing deformation at atomistic to nanometer length scales has prevented a clear mechanistic understanding of nanoscale …
Successfully Navigating The Early Years Of A Faculty Position, Michelle L. Kovarik, Christopher R. Harrison, Thomas J. Wenzel
Successfully Navigating The Early Years Of A Faculty Position, Michelle L. Kovarik, Christopher R. Harrison, Thomas J. Wenzel
Faculty Scholarship
No abstract provided.