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Full-Text Articles in Physical Sciences and Mathematics
Relating Dynamic Protein Interactions Of Metallochaperones With Metal Transfer At The Single-Molecule Level, J. J. Benítez, A. M. Keller, D. L. Huffman, Liliya A. Yatsunyk, A. C. Rosenzweig, P. Chen
Relating Dynamic Protein Interactions Of Metallochaperones With Metal Transfer At The Single-Molecule Level, J. J. Benítez, A. M. Keller, D. L. Huffman, Liliya A. Yatsunyk, A. C. Rosenzweig, P. Chen
Chemistry & Biochemistry Faculty Works
Metallochaperones undertake specific interactions with their target proteins to deliver metal ions inside cells. Understanding how these protein interactions are coupled with the underlying metal transfer process is important, but challenging because they are weak and dynamic. Here we use a nanovesicle trapping scheme to enable single-molecule FRET measurements of the weak, dynamic interactions between the copper chaperone Hahl and the fourth metal binding domain (MBD4) of WDP. By monitoring the behaviors of single interacting pairs, we visualize their interactions in real time in both the absence and the presence of various equivalents of Cu(1+). Regardless of the proteins' metallation …
Reactive Quenching Of Od A (2)Σ(+) By H-2: Translational Energy Distributions For H- And D-Atom Product Channels, J. H. Lehman, Jesse Lyle Bertrand , '11, Thomas Alex Stephenson, M. I. Lester
Reactive Quenching Of Od A (2)Σ(+) By H-2: Translational Energy Distributions For H- And D-Atom Product Channels, J. H. Lehman, Jesse Lyle Bertrand , '11, Thomas Alex Stephenson, M. I. Lester
Chemistry & Biochemistry Faculty Works
The H- and D-atom products from collisional quenching of OD A (2)Sigma(+) by H-2 are characterized through Doppler spectroscopy using two-photon (2 S-2 <-<- 1 S-2) laser-induced fluorescence. Partial deuteration enables separation of the channel forming H + HOD products, which accounts for 75% of reactive quenching events, from the D + H2O product channel. The Doppler profiles, along with those reported previously for other isotopic variants, are transformed into product translational energy distributions using a robust fitting procedure based on discrete velocity basis functions. The product translational energy distribution for the H- atom channel is strongly peaked at low energy (below 0.5 eV) with a long tail extending to the energetic limit. By contrast, the D-atom channel exhibits a small peak at low translational energy with a distinctive secondary peak at higher translational energy (approximately 1.8 eV) before falling off to higher energy. In both cases, most of the available energy flows into internal excitation of the water products. Similar distributions are obtained upon reanalysis of D- and H- atom Doppler profiles, respectively, from reactive quenching of OH A (2)Sigma(+) by D-2. The sum of the translational energy distributions for H- and D- atom channels is remarkably similar to that obtained for OH A (2)Sigma(+) + H-2, where the two channels cannot be distinguished from one another. The product translational energy distributions from reactive quenching are compared with those obtained from a previous experiment performed at higher collision energy, quasiclassical trajectory calculations of the post-quenching dynamics, and a statistical model. (C) 2011 American Institute of Physics. [doi: 10.1063/1.3644763]
Experimental Characterization Of The Weakly Anisotropic Cn X(2)Σ(+) + Ne Potential From Ir-Uv Double Resonance Studies Of The Cn-Ne Complex, J. M. Beames, B. A. O'Donnell, M. Ting, M. I. Lester, Thomas Alex Stephenson
Experimental Characterization Of The Weakly Anisotropic Cn X(2)Σ(+) + Ne Potential From Ir-Uv Double Resonance Studies Of The Cn-Ne Complex, J. M. Beames, B. A. O'Donnell, M. Ting, M. I. Lester, Thomas Alex Stephenson
Chemistry & Biochemistry Faculty Works
IR-UV double resonance spectroscopy has been used to characterize hindered internal rotor states (n(K) = 0(0), 1(1), and 1(0)) of the CN-Ne complex in its ground electronic state with various degrees of CN stretch (nu(CN)) excitation. Rotationally resolved infrared overtone spectra of the CN-Ne complex exhibit perturbations arising from Coriolis coupling between the closely spaced hindered rotor states (1(1) and 1(0)) with two quanta of CN stretch (nu(CN) = 2). A deperturbation analysis is used to obtain accurate rotational constants and associated average CN center-of-mass to Ne separation distances as well as the coupling strength. The energetic ordering and spacings …
Dicarbonyldichloridobis(Trimethylphosphane)Iron(Ii)–Carbonyldichloridotris(Trimethylphosphane)Iron(Ii)–Tetrahydrofuran (1/1/2), Nigam Rath, Meghan Stouffer, Matthew Janssen, John Bleeke
Dicarbonyldichloridobis(Trimethylphosphane)Iron(Ii)–Carbonyldichloridotris(Trimethylphosphane)Iron(Ii)–Tetrahydrofuran (1/1/2), Nigam Rath, Meghan Stouffer, Matthew Janssen, John Bleeke
Chemistry & Biochemistry Faculty Works
The asymmetric unit of the title crystal, [FeCl2(C3H9P)3(CO)]·[FeCl2(C3H9P)2(CO)2]·2C4H8O, contains half molecules of the two closely related FeII complexes lying on mirror planes and a tetrahydrofuran solvent molecule, one C atom of which is disordered over two sets of sites with site occupancy factors 0.633 (9) and 0.367 (9). In both FeII complex molecules, a distorted octahedral coordination geometry has been observed around the Fe atoms. Weak intermolecular C-H...O interactions are observed in the crystal structure.
Rudimentary G-Quadruplex-Based Telomere Capping In Saccharomyces Cerevisiae, J. S. Smith, Q. Chen, Liliya A. Yatsunyk, John Michael Nicoludis , '12, M. S. Garcia, R. Kranaster, S. Balasubramanian, D. Monchaud, M.-P. Teulade-Fichou, L. Abramowitz, D. C. Schultz, F. B. Johnson
Rudimentary G-Quadruplex-Based Telomere Capping In Saccharomyces Cerevisiae, J. S. Smith, Q. Chen, Liliya A. Yatsunyk, John Michael Nicoludis , '12, M. S. Garcia, R. Kranaster, S. Balasubramanian, D. Monchaud, M.-P. Teulade-Fichou, L. Abramowitz, D. C. Schultz, F. B. Johnson
Chemistry & Biochemistry Faculty Works
Telomere capping conceals chromosome ends from exonucleases and checkpoints, but the full range of capping mechanisms is not well defined. Telomeres have the potential to form G-quadruplex (G4) DNA, although evidence for telomere G4 DNA function in vivo is limited. In budding yeast, capping requires the Cdc13 protein and is lost at nonpermissive temperatures in cdc13-1 mutants. Here, we use several independent G4 DNA-stabilizing treatments to suppress cdc13-1 capping defects. These include overexpression of three different G4 DNA binding proteins, loss of the G4 DNA unwinding helicase Sgs1, or treatment with small molecule G4 DNA ligands. In vitro, we show …