Physical Sciences and Mathematics Commons^™

Methodological Approach To The Ex Vivo Expansion And Detection Of T. Cruzi-Specific T Cells From Chronic Chagas Disease Patients, Gonzalo R. Acevedo, Silvia A. Longhi, Alcinette Bunying, Nazila Sabri, Augusto Atienza, Maria P. Zago, Radleigh Santos, Valeria A. Judkowski, Clemencia Pinilla, Karina A. Gomez

Mathematics Faculty Articles

The discovery of T cell epitopes is essential not only for gaining knowledge about host response to infectious disease but also for the development of immune-intervention strategies. In Chagas disease, given the size and complexity of the Trypanosoma cruzi proteome and its interaction with the host’s immune system, the fine specificity of T cells has not been extensively studied yet, and this is particularly true for the CD4+ T cell compartment. The aim of the present work was to optimize a protocol for the generation of parasite-specific memory T cell lines, representative of their in vivo precursor populations and capable …