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Full-Text Articles in Physical Sciences and Mathematics
Exploring Indicator Displacement Assays For Phosphate Detection In Seawater, Francis Radics
Exploring Indicator Displacement Assays For Phosphate Detection In Seawater, Francis Radics
Chemistry & Biochemistry Student Scholarship
Francis Radics ’22
Major: Biochemistry
Faculty Mentor: Dr. John Breen, Chemistry and Biochemistry
Indicator displacement assays are based on the optical signal modulation of a noncovalently bound indicator upon dissociation by an analyte species. Our work has focused on exploring the lower detection limits for luminescent displacement assays for inorganic phosphate in seawater using complex ions containing two di(2-picolyl)amine ligands (also called DPA or bis(2-pyridylmethyl)amine), each coordinating a zinc cation. Following the work of B.D. Smith and coworkers, we have prepared three ligands by covalently attaching two DPA moieties, 2,6-bis(chloromethyl) benzene, and 2,6-bis(chloromethyl)-4-methylphenol, and 1,2-phenylenedimethylamine, for assays with 6,7-dihydroxy-4-methanesulfonic acid …
Towards The Development Of Low-Cost And Easily-Deployable Sensing Platforms For Phosphate, Maureen Pontarelli, Thomas Koch
Towards The Development Of Low-Cost And Easily-Deployable Sensing Platforms For Phosphate, Maureen Pontarelli, Thomas Koch
Chemistry & Biochemistry Student Scholarship
Maureen Pontarelli ’20
Major: Chemistry
Thomas Koch '20
Major: Biochemistry
Faculty Mentor: Dr. John Breen, Chemistry and Biochemistry
Exploration Of K2 Mycobacteriophages With Bred, Colby Agostino, Olivia Schmitt
Exploration Of K2 Mycobacteriophages With Bred, Colby Agostino, Olivia Schmitt
Chemistry & Biochemistry Student Scholarship
Colby Agostino ’22
Major: Biochemistry and Computer Science
Olivia Schmitt ’22
Major: Biochemistry
Faculty Mentor: Dr. Kathleen A. Cornely, Chemistry and Biochemistry
ZoeJ and Marcoliusprime are mycobacteriophage capable of infecting tuberculosis causing bacteria via the lytic and lysogenic cycles. The lytic cycle is the form of viral replication that results in cell death. Lysogeny involves phage DNA being integrated with the DNA of the host cell and preserved through binary fission. However, the bacteria causing tuberculosis do not die in the lysogenic phase. This research project will involve creating mutants of ZoeJ and Marcoliusprime that will not contain the genes …
Mycobacteriophage Morphology As A Diagnostic For Cluster Assignment, Ethan Dionne
Mycobacteriophage Morphology As A Diagnostic For Cluster Assignment, Ethan Dionne
Chemistry & Biochemistry Student Scholarship
Major: Biochemistry
Faculty Mentor: Dr. Kathleen A. Cornely, Chemistry and Biochemistry
Phages are viral bodies that infect bacterial hosts, and have shown promising applications as alternatives to antibiotics for the treatment of bacterial infections. This project examines the morphology of siphoviridae mycobacteriophage, which have long, flexible, non-contractile tails as well as the characteristic head, called a capsid. Using electron microscopy photos of sequenced phages, tail length and capsid diameter were measured and compared to further characterize morphological relationships between genetically distinct phages. The data presented has the potential to work as a diagnostic tool to classify unsequenced phages to genetically …
Surface Mutation Thr34his Facilitates Purification Of Haemophilus Influenza Carbonic Anhydrase Via Metal Affinity Chromatography, Caroline Foley, Dr. Kathleen Cornely
Surface Mutation Thr34his Facilitates Purification Of Haemophilus Influenza Carbonic Anhydrase Via Metal Affinity Chromatography, Caroline Foley, Dr. Kathleen Cornely
Chemistry & Biochemistry Student Scholarship
In order to pursue Haemophilus influenza carbonic anhydrase (HICA) as a potential drug target, easy and efficient purification methods must be developed. While immobilized metal affinity chromatography (IMAC) may be used, complications with polyhistidine tags is a concern. Inspired by the endogenous metal affinity of Escherichia coli β-carbonic anhydrase (ECCA), we suggest that the generation of histidine clusters on HICA’s surface will facilitate its purification by metal affinity chromatography without the potential interference of His-tags. Here we investigate the Thr34His mutation as a method to generate metal affinity in HICA. Since Thr34His is located only 5.3 Å away from His32, …