Physical Sciences and Mathematics Commons^™

Substrate Analogs For Characterizing The Substrate Tolerance Of S. Pneumoniae Srta, Orion Banks

Graduate Student Symposium

Bacterial sortases have been widely studied for their usefulness in protein modification, however, the variable substrate specificity and activity between homologs of these enzymes is not yet fully characterized. To attempt to further understand sorting signal recognition, we are working towards a substrate bound structure of sortase A from Streptococcus pneumoniae (SrtA_pneu). This enzyme displays a wide tolerance for alternate amino acids within the canonical LPXTG sorting motif. Our strategy involves a non-cleavable substrate analog that can be docked into the active site, allowing for elucidation of a structure displaying the key contacts that allow the enzyme …