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Full-Text Articles in Physical Sciences and Mathematics
Measurement Of Anti-Cancer Agent Methoxyamine In Plasma By Tandem Mass Spectrometry With On-Line Sample Extraction, Shuming Yang, Lili Liu, Stanton L. Gerson, Yan Xu
Measurement Of Anti-Cancer Agent Methoxyamine In Plasma By Tandem Mass Spectrometry With On-Line Sample Extraction, Shuming Yang, Lili Liu, Stanton L. Gerson, Yan Xu
Chemistry Faculty Publications
In this work, we present the development and validation of a tandem mass spectrometry method for the quantitative determination of methoxyamine (CH3ONH2), a potential new chemotherapeutic agent, in human and mouse plasma. Methoxyamine together with the internal standard (I.S.) methoxyl-D3-amine was directly derivatized in plasma sample with a novel chemical agent 4-(N,N-diethylamino)benzaldehyde. The product solution was injected into an on-line Oasis® HLB extraction column ( mm) for analyte extraction. After the elution of extractives, the derivatized analytes were monitored by the positive-electrospray-ionization mass spectrometry (ESI-MS-MS). The structures of derivatized analytes were elucidated by fragmentation. Quantitation of plasma methoxyamine was carried …
Measurement Of The Anti-Cancer Agent Gemcitabine In Human Plasma By High-Performance Liquid Chromatography, Bruce Keith, Yan Xu, Jean L. Grem
Measurement Of The Anti-Cancer Agent Gemcitabine In Human Plasma By High-Performance Liquid Chromatography, Bruce Keith, Yan Xu, Jean L. Grem
Chemistry Faculty Publications
A reversed-phase HPLC assay has been developed to determine the concentration of the anti-metabolite 2′,2′-difluorodeoxycytidine (gemcitabine, dFdC) in human plasma over the concentration range of 0.5–150 μM (0.13–39.44 μg/ml), and 2′,2′-difluorodeoxyuridine (dFdU), the deaminated, inactive metabolite, over the range of 1.0–227 μM (0.26–60 μg/ml). After the addition of 20 nmol 2′-fluorodeoxycytidine (FdC) as an internal standard, 0.5-ml samples of plasma were subjected to acetonitrile precipitation, followed by analysis using a gradient reversed-phase HPLC assay with UV detection. A Phenomenex Columbus™ C18 column, 5 μm, 150×4.6 mm, and a Waters C18, 4 μm, Nova-Pak Sentry guard column were used to achieve …
Liquid Chromatography–Mass Spectrometry Method For The Analysis Of The Anti-Cancer Agent Capecitabine And Its Nucleoside Metabolites In Human Plasma, Yan Xu, Jean L. Grem
Liquid Chromatography–Mass Spectrometry Method For The Analysis Of The Anti-Cancer Agent Capecitabine And Its Nucleoside Metabolites In Human Plasma, Yan Xu, Jean L. Grem
Chemistry Faculty Publications
A reversed-phase high-performance liquid chromatography method with electrospray ionization and mass spectral detection is described for the determination of capecitabine, 5′-deoxy-5-fluorocytidine and 5′-deoxy-5-fluorouridine in human plasma with 5-chloro-2′-deoxyuridine as the internal standard. An on-line sample clean-up procedure allows dilution of the plasma sample with the initial mobile phase. The linear dynamic range is 0.0500–10.0 μg/ml for capecitabine, and 0.0500–25.0 μg/ml for the metabolites, 5′-deoxy-5-fluorocytidine and 5′-deoxy-5-fluorouridine, respectively. This method has been used to analyze plasma samples from patients receiving capecitabine in combination with oxaliplatin.
Possible Cobalt-Cobalt Bridging By A Hemiacetal In The Dinuclear Cobalt Complex Bearing The Ligand Bis(3-(2-Pyridylmethyleneamino)Phenyl) Sulfone, Armando M. Guidote Jr, Tomoko Imajo, Yasuhiko Kurusu, Yoshiro Masuyama
Possible Cobalt-Cobalt Bridging By A Hemiacetal In The Dinuclear Cobalt Complex Bearing The Ligand Bis(3-(2-Pyridylmethyleneamino)Phenyl) Sulfone, Armando M. Guidote Jr, Tomoko Imajo, Yasuhiko Kurusu, Yoshiro Masuyama
Chemistry Faculty Publications
A dinuclear cobalt complex bearing the ligand bis(3-(2-pyridylmethyleneamino)phenyl) sulfone (BPMAPS) was prepared. It is proposed that the structure of this is [Coz(BPMAPS)(m-0AcMhemi-Et)]PF6 wherein the cobalt centers are bridged by two carboxylato groups in m-fashion and a hemiacetal with an ethoxy group (hemi-Et). This proposal is based on the similarity of the FT-IR, UV-Vis, and FAB-MS results with the crystallographically characterized dinuclear manganese complex [Mn2(BPMAPS)(m-OAcMhemi-Me)]PF6, and elemental analysis results.