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Full-Text Articles in Physical Sciences and Mathematics
Gas-Phase Proton Affinities For Twenty Of The Proline-Containing Dipeptides, Henry Cardwell
Gas-Phase Proton Affinities For Twenty Of The Proline-Containing Dipeptides, Henry Cardwell
Undergraduate Honors Theses
Peptide fragmentation plays a crucial role in the analysis of proteins through mass spectrometry-based proteomics. Most proteomics experiments take place in the low-energy regime and are governed by the mobile proton model which predicts random cleavages along the peptide backbone; however, there sometimes arise circumstances where the mobile proton model fails causing sequencing algorithms to misidentify peptides. One such example is noted in the “proline effect” wherein proline-containing peptides preferentially fragment N-terminal. While it has been established that the “proline effect” is due to the rigidity and basicity of the proline N-terminus, a further understanding of the factors influencing the …
Chemical Analysis Of Organic Compounds In Dew Water, Monica Dibley
Chemical Analysis Of Organic Compounds In Dew Water, Monica Dibley
Undergraduate Honors Theses
Water films on outdoor surfaces, such as dew, can act as a reservoir for organic molecules deposited from the atmosphere and they present a potential reactive medium for chemical transformations. To better understand the flux of volatile organic compounds from evaporating films, the composition and reactivity of the complex mixture of dissolved organic material (DOM) found in these films need to be characterized. Previous studies have measured the salts and the small organic molecules in dew collected on clean Teflon surfaces or condensers. Here, we expand on this by probing the organic chemicals found on natural outdoor surfaces covered in …
Differential Protein Expression In Bacteriophages Crimd And Larva, Daria Moody
Differential Protein Expression In Bacteriophages Crimd And Larva, Daria Moody
Undergraduate Honors Theses
Proteomics studies allow us to answer questions about differential protein expression across different systems. Mass spectrometry is a powerful tool in these studies due to the distinct masses of the amino acids that compose proteins. In our experiment, we used a bottom-up approach and focused on two bacteriophages found on the William & Mary campus, CrimD and Larva. The infection of Mycobacterium smegmatis, a nonpathogenic model for tuberculosis, by these two bacteriophages was frozen at five different timepoints, and our goal was to compare the differential protein expression across the samples in order to gain a greater understanding of …
Characterization Of Indoor Surface Films By High-Resolution Mass Spectrometry, Emily Nicole Legaard
Characterization Of Indoor Surface Films By High-Resolution Mass Spectrometry, Emily Nicole Legaard
Dissertations, Theses, and Masters Projects
Atmospheric chemistry has primarily focused on outdoor environments for many decades. Plenty of models based on the findings of outdoor atmospheric researchers have been built to deepen the understanding of outdoor environments and predict how certain changes in specific parameters may affect the overall atmosphere. The same cannot be said for indoor environments, which remain relatively unknown despite the fact that humans in developed countries spend the vast majority of their lives in indoor environments. This thesis seeks to provide an early look at the makeup of indoor surface films, specifically at their molecular compositions and thicknesses. These characteristics are …