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Capillary Electrophoresis: Method Development And Application In Biomolecule Analysis, Lindsay Marie Veltri
Capillary Electrophoresis: Method Development And Application In Biomolecule Analysis, Lindsay Marie Veltri
Graduate Theses, Dissertations, and Problem Reports
Abstract
Capillary Electrophoresis: Method Development and Application in Biomolecule Analysis
Lindsay Veltri
Biomolecule analysis is essential in disease diagnostics and the development of therapeutic agents. There is a vast array of methods available for biomolecule analysis, however, capillary electrophoresis is well-suited due to minimal sample consumption (~nanoliters of solution) and fast analysis times. In this report, capillary electrophoresis was used to analyze glycosylation of therapeutic proteins. Additionally, a capillary electrophoresis-mass spectrometry method is reported for the analysis of proteins.
Post-translation protein modifications like glycosylation, play an important in protein structure and function. Modifications of protein glycosylation can lead to improved …
Capillary Electrophoresis Analysis Of Proteins Using Phospholipid Based Materials, Cassandra L. Crihfield
Capillary Electrophoresis Analysis Of Proteins Using Phospholipid Based Materials, Cassandra L. Crihfield
Graduate Theses, Dissertations, and Problem Reports
Capillary electrophoresis has many advantages that make it a powerful technique for the analysis of proteins, which is challenging due to the natural heterogeneity of proteins. The small sample volume required for each analysis (nano- and pico-liter), fast separation times (<30 >min), and ability to couple the separation to mass spectrometry are all factors that make capillary electrophoresis an ideal technology for protein separations. Capillary zone electrophoresis and capillary gel electrophoresis are the two most commonly employed modes for protein analysis and allow for rapid, high resolution separations that can be automated. This makes capillary electrophoresis ideal for meeting the …30>