Physical Sciences and Mathematics Commons^™

Studies Toward The Development Of A Microelectrode Array For Detection Of Dopamine Through Redox Cycling, Anupama Aggarwal

Graduate Theses and Dissertations

Redox cycling is an electrochemical technique that cycles the reversible redox species between its oxidative states repeatedly on generator and collector electrodes. Two or more individually-addressable microelectrodes located close to each other allow redox cycling to be possible. Electrochemical behavior of a biologically important molecule, dopamine is examined under redox cycling conditions. To our knowledge, this is the first report on detection of physiological concentration of dopamine in presence of up to 100 times excess ascorbate with the use of redox cycling, without the involvement of polymer coating such as Nafion®.

Microfabrication was used to produce different geometries (parallel bands …

Visualizing The Spatial Localization Of Active Matrix Metalloproteinases (Mmps) Using Maldi Imaging Ms, Sasirekha Muruganantham

Graduate Theses and Dissertations

Biomaterial implantation induces the foreign body response (FBR). Development of longer-term implants relies on the thorough understanding of the FBR. The progression of the FBR is regulated by a number of biomolecules including cytokines, chemokines, and matrix metalloproteinases (MMPs). The nature of the FBR requires the spatial and temporal regulation of these mediators. MMPs are an extremely large and diverse group of enzymes that play key roles in regulating the FBR. Precise spatiotemporal regulation of MMPs defines their proteolytic activities. The aim of this project is to develop a new bioanalytical method to visualize the localization of active MMPs at …

Dynamics And Catalytic Resolution Of Selected Chiral Organolithiums, Timothy Kum Beng

Graduate Theses and Dissertations

One of the most important developments of the last decade has been the emergence of new methods to dynamically resolve racemic organolithiums using stoichiometric amounts of the chiral ligand. When this concept is implemented successfully, it obviates the need for covalently attached chiral auxiliary based methods, asymmetric deprotonation, and asymmetric synthesis of a precursor stannane as ways to access enantioenriched organolithium compounds for use in asymmetric synthesis. Since certain electrophiles consume the chiral ligand, it is desirable to render this process catalytic in the chiral ligand.

As part of a larger study on the amenability of chiral organolithiums to a …