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Full-Text Articles in Medicine and Health Sciences
A New Role For A Snare Protein As A Regulator Of The Ypt7/Rab-Dependent Stage Of Docking, Christian Ungermann, Albert Price, William Wickner
A New Role For A Snare Protein As A Regulator Of The Ypt7/Rab-Dependent Stage Of Docking, Christian Ungermann, Albert Price, William Wickner
Dartmouth Scholarship
The homotypic fusion of yeast vacuoles occurs in an ordered cascade of priming, docking, and fusion. The linkage between these steps has so far remained unclear. We now report that Vam7p (the vacuolar SNAP-23/25 homolog) signals from the cis-SNARE complex to Ypt7p (the vacuolar Rab/Ypt) to initiate the docking process. After Vam7p has been released from the cis-SNARE complex by Sec18p-mediated priming, it is still required for Ypt7p-dependent docking and it needs Ypt7p to remain on the vacuole.
Proteins Needed For Vesicle Budding From The Golgi Complex Are Also Required For The Docking Step Of Homotypic Vacuole Fusion, Albert Price, William Wickner, Christian Ungermann
Proteins Needed For Vesicle Budding From The Golgi Complex Are Also Required For The Docking Step Of Homotypic Vacuole Fusion, Albert Price, William Wickner, Christian Ungermann
Dartmouth Scholarship
Vam2p/Vps41p is known to be required for transport vesicles with vacuolar cargo to bud from the Golgi. Like other VAM-encoded proteins, which are needed for homotypic vacuole fusion, we now report that Vam2p and its associated protein Vam6p/Vps39p are needed on each vacuole partner for homotypic fusion. In vitro vacuole fusion occurs in successive steps of priming, docking, and membrane fusion. While priming does not require Vam2p or Vam6p, the functions of these two proteins cannot be fulfilled until priming has occurred, and each is required for the docking reaction which culminates in trans-SNARE pairing. Consistent with their dual function …
A Cultivated Taste For Yeast., C Brenner
A Cultivated Taste For Yeast., C Brenner
Kimmel Cancer Center Faculty Papers
The availability of complete genomic sequences of Saccharomyces cerevisiae has catalyzed a cultural change in the practice of yeast biology, providing opportunities to develop high throughput techniques to define protein function, to define drug targets, and to discover and characterize drugs.