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Full-Text Articles in Medicine and Health Sciences
Tricarboxylic Acid Cycle And One-Carbon Metabolism Pathways Are Important In Edwardsiella Ictaluri Virulence., Neeti Dahal, Hossam Abdelhamed, Jingjun Lu, Attila Karsi, Mark L. Lawrence
Tricarboxylic Acid Cycle And One-Carbon Metabolism Pathways Are Important In Edwardsiella Ictaluri Virulence., Neeti Dahal, Hossam Abdelhamed, Jingjun Lu, Attila Karsi, Mark L. Lawrence
College of Veterinary Medicine Publications and Scholarship
Edwardsiella ictaluri is a Gram-negative facultative intracellular pathogen causing enteric septicemia of channel catfish (ESC). The disease causes considerable economic losses in the commercial catfish industry in the United States. Although antibiotics are used as feed additive, vaccination is a better alternative for prevention of the disease. Here we report the development and characterization of novel live attenuated E. ictaluri mutants. To accomplish this, several tricarboxylic acid cycle (sdhC, mdh, and frdA) and one-carbon metabolism genes (gcvP and glyA) were deleted in wild type E. ictaluri strain 93-146 by allelic exchange. Following bioluminescence tagging of the E. ictaluri ?sdhC, ?mdh, …