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Full-Text Articles in Medicine and Health Sciences
Evidence Of Kisspeptin Receptor Expression In Gnrh Neurons In The Preoptic Area And Arcuate Hypothalamic Nuclei In Cycling Buffaloes, Thuchadaporn Chaikhun-Marcou, Pongsiwa Sotthibandhu, Victoria Kyle, Shel Hwa Yeo, William Henry Colledge, Siriwat Suadsong
Evidence Of Kisspeptin Receptor Expression In Gnrh Neurons In The Preoptic Area And Arcuate Hypothalamic Nuclei In Cycling Buffaloes, Thuchadaporn Chaikhun-Marcou, Pongsiwa Sotthibandhu, Victoria Kyle, Shel Hwa Yeo, William Henry Colledge, Siriwat Suadsong
The Thai Journal of Veterinary Medicine
Our previous study reported the expression of kisspeptin in the preoptic area (POA) and arcuate nucleus (ARC) of the hypothalamus of cycling buffalo cows. In ruminants, POA and ARC are the main hypothalamic nuclei through which kisspeptin acts on the hypothalamic-pituitary-gonadal axis, due to their stimulation of gonadotropin releasing hormone (GnRH) secretion. In many species, kisspeptin activates GnRH neurons via its receptors (KISS1R or GPR54). The present study was designed to explore the expression of KISS1R by GnRH neurons in the POA and ARC hypothalamic nuclei in buffalo cow brains by double-labeling immunohistochemistry. In both the POA and ARC areas, …
Trophoblast Major Histocompatibility Complex Class I Expression Is Associated With Immune-Mediated Rejection Of Bovine Fetuses Produced By Cloning, Heloisa M. Rutigliano, Aaron J. Thomas, Amanda Wilhelm, Benjamin R. Sessions, Brady A. Hicks, Donald H. Schlafer, Kenneth L. White, Christopher J. Davies
Trophoblast Major Histocompatibility Complex Class I Expression Is Associated With Immune-Mediated Rejection Of Bovine Fetuses Produced By Cloning, Heloisa M. Rutigliano, Aaron J. Thomas, Amanda Wilhelm, Benjamin R. Sessions, Brady A. Hicks, Donald H. Schlafer, Kenneth L. White, Christopher J. Davies
Animal, Dairy, and Veterinary Science Faculty Publications
Trophoblast cells from bovine somatic cell nuclear transfer (SCNT) conceptuses express major histocompatibility complex class I (MHC-I) proteins early in gestation, and this may be one cause of the significant first-trimester embryonic mortality observed in these pregnancies. MHC-I homozygous-compatible (n = 9), homozygous-incompatible (n = 8), and heterozygous-incompatible (n = 5) SCNT pregnancies were established. The control group consisted of eight pregnancies produced by artificial insemination. Uterine and placental samples were collected on Day 35 ± 1 of pregnancy, and expression of MHC-I, leukocyte markers, and cytokines were examined by immunohistochemistry. Trophoblast cells from all SCNT pregnancies expressed MHC-I, while …
Involucrin Expression And Association With Ki-67 In Paraffin Embedded Tissue Of Canine Skin Tumors: A Retrospective Study, Nongnut Assawawongkasem, Gunnaporn Suriyaphol, Sayamon Srisuwattanasagul, Sirin Theerawatanasirikul, Achariya Sailasuta
Involucrin Expression And Association With Ki-67 In Paraffin Embedded Tissue Of Canine Skin Tumors: A Retrospective Study, Nongnut Assawawongkasem, Gunnaporn Suriyaphol, Sayamon Srisuwattanasagul, Sirin Theerawatanasirikul, Achariya Sailasuta
The Thai Journal of Veterinary Medicine
Involucrin (INV) is a cornified envelope precursor protein that is commonly used as an early stage marker of human terminal keratinocyte differentiation. This research investigated the expression of INV in canine skin tumors using 56 formalin-fixed paraffin-embedding (FFPE) biopsied specimens. The expression of INV was investigated by immunohistochemistry (IHC) and RT-PCR. Correlation between INV and Ki-67 was also performed. The malignant tumor groups showed significantly different INV staining scores and Ki-67 positive cells than the normal skin group by IHC (p<0.05). The INV scores of moderately differentiated SCC (MSCC), trichoblastoma (TCB), basal cell carcinoma (BCC) and poorly differentiated SCC (PSCC) significantly decreased, with a statistically significant difference (p<0.05). Interestingly, the INV of poorly differentiated SCC disappeared in some specimens. The INV expression in most tumors was found to be inversely proportionate to Ki-67 positive cells. In addition, only SCC regardless of tumor grade without tumor-graded specimens showed an inversely statistically significant difference between the Ki-67 positive cells and INV expression (p<0.0001). In RT-PCR, mRNA expression of the FFPE specimens showed substantial similarity of INV expression as in IHC. It is suggested that the INV expression should be further studied in terms of clinical prognosis in canine cutaneous SCC.
The Effects Of Acidified Pepsin On Porcine Vocal Fold Tissue: Developing A Porcine Model Of Laryngopharyngeal Reflux Disease, Abigail Cox Durkes
The Effects Of Acidified Pepsin On Porcine Vocal Fold Tissue: Developing A Porcine Model Of Laryngopharyngeal Reflux Disease, Abigail Cox Durkes
Open Access Dissertations
Approximately 7.5 million Americans are affected by a voice disorder. In the last year, 7.2% of people missed one or more days of work due to a voice problem. For professional voice users, such as teachers, the rate increases to 20% and an annual cost of $2.5 billion. Voice disorders are complex and multi-faceted, as well as difficult to diagnose and treat. Prospective animal studies are necessary to study the pathophysiology of voice disorders, optimize our understanding of laryngeal pathology, and improve treatment outcomes. The pig provides a unique opportunity to test hypotheses relating to laryngeal disease because porcine vocal …
Mast Cell Typing In Aortic Onchocerciasis And The Immunohistochemicaldemonstration Of Wolbachia Bacteria, Rahşan Yilmaz, Melek Koçak, Zafer Özyildiz, İsmai̇l Şah Harem
Mast Cell Typing In Aortic Onchocerciasis And The Immunohistochemicaldemonstration Of Wolbachia Bacteria, Rahşan Yilmaz, Melek Koçak, Zafer Özyildiz, İsmai̇l Şah Harem
Turkish Journal of Veterinary & Animal Sciences
In this study, the presence of endosymbiotic Wolbachia bacteria was determined by immunohistochemical methods in 8 out of 43 paraffin-embedded bovine aortic tissues that were macroscopically and microscopically diagnosed with aortic onchocerciasis in the archive of the Harran University Faculty of Veterinary Medicine's Department of Pathology. By means of the enzyme histochemical and immunohistochemical staining methods, it was ascertained that the tryptase-containing subtype of mast cells (MCT) was involved in the inflammatory response in the bovine aortae with onchocerciasis. The aim of this study was to elucidate the pathogenesis of aortic onchocerciasis lesions caused by Onchocerca armillata in Turkey and …
Immunohistochemical Evaluation Of Canine And Felinemerkel Cell Tumors?A Report Of Two Cases, Mateusz Mikiewicz, Iwona Otrocka-Domagala, Katarzyna Pazdzior-Czapula, Michal Gesek, Anna Mikolajczyk, Agnieszka Blacha
Immunohistochemical Evaluation Of Canine And Felinemerkel Cell Tumors?A Report Of Two Cases, Mateusz Mikiewicz, Iwona Otrocka-Domagala, Katarzyna Pazdzior-Czapula, Michal Gesek, Anna Mikolajczyk, Agnieszka Blacha
Turkish Journal of Veterinary & Animal Sciences
Merkel cell tumors, a type of cutaneous round-cell tumors, are rarely observed in dogs and cats. This tumor type originates from cutaneous neuroendocrine cells. In the present study, the morphology of canine and feline Merkel cell tumors and their immunoexpression of neuron-specific enolase, cytokeratin, chromogranin A, Ki67, and metallothionein were evaluated using histopathological and immunohistochemical methods. The canine Merkel cell tumor displayed a diffuse morphological pattern, whereas the feline tumor displayed a solid morphological pattern. Both tumors were positive for neuron-specific enolase and cytokeratin, but negative for chromogranin A. The Ki67 indexes in the canine and feline tumors were 7% …