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Full-Text Articles in Medicine and Health Sciences
Comparison Of The Efficiency Of Concentrated Soluble Recombinant Phospholipase D And Natural Phospholipase D Enzymes, Asli Sakmanoğlu, Osman Ergani̇ş
Comparison Of The Efficiency Of Concentrated Soluble Recombinant Phospholipase D And Natural Phospholipase D Enzymes, Asli Sakmanoğlu, Osman Ergani̇ş
Turkish Journal of Veterinary & Animal Sciences
Phospholipase D (PLD) is a major virulence determinant of Corynebacterium pseudotuberculosis. Various studies have focused on the expression of recombinant PLD (rPLD) enzyme in different Escherichia coli strains, but generally a high yield of insoluble protein was reported. The aims of this study were to express soluble rPLD by different methods in E. coli. The rPLD and natural PLD (dPLD) enzymes were concentrated using an ultramembrane cassette system after the efficiencies of these concentrated enzymes were compared. The rPLD enzyme was expressed in One Shot®BL21(DE3) E. coli when induced by IPTG in TY medium. Soluble dPLD and rPLD enzyme hemolytic …
Gross Responses And Apparent Ileal Digestibility Of Amino Acids And Minerals In Broiler Chicken Fed Vegetable-Based Starter Diets Supplemented With Microbial Enzymes, Mohammad Abul Hossain, Paul Ade Iji, Al-Farooq Mohammad Fakhrul Islam
Gross Responses And Apparent Ileal Digestibility Of Amino Acids And Minerals In Broiler Chicken Fed Vegetable-Based Starter Diets Supplemented With Microbial Enzymes, Mohammad Abul Hossain, Paul Ade Iji, Al-Farooq Mohammad Fakhrul Islam
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to investigate the effects of diet and exogenous enzymes on growth responses and micronutrient digestibility of broilers. Day-old broilers (n = 256) were distributed into a 2 x 2 factorial design and were fed on two basal diets: soybean (T1) or canola (T2) meals as such, or supplemented with enzymes up to 21 days. Feed intake (FI) and live weight (LW) to 21 days on the T2 diet was higher (P < 0.001) than that on T1. Feed conversion ratio (FCR) was better (P < 0.01) on T1 than on T2 at 21 days. Enzymes generally improved (P < 0.001) FI, FCR, and LW. The digestibility of histidine was higher (P < 0.05) on T2, but lysine digestibility was higher (P < 0.01) on T1 at 21 days. Histidine, threonine, lysine, valine, isoleucine, and phenylalanine digestibility was improved (P < 0.05) in the supplemented diets. Mineral digestibility was unaffected (P > 0.05) by diet, but enzymes increased (P < 0.05) digestibility of P, K, Mn, and Cu at 21 days. The digestibility of Cu, Zn, and Mg was higher (P < 0.05) on T2, whereas Ca digestibility was greater (P < 0.05) on the T1 diet. Broilers' growth responded positively to enzyme diets, probably due to improvement in nutrient digestibility.