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Full-Text Articles in Medicine and Health Sciences
Caffeic Acid Phenethyl Ester Protects Lung Alveolar Epithelial Cellsfrom Cigarette Smoke-Induced Damage, Firat Bariş Barlas, Suat Erdogan
Caffeic Acid Phenethyl Ester Protects Lung Alveolar Epithelial Cellsfrom Cigarette Smoke-Induced Damage, Firat Bariş Barlas, Suat Erdogan
Turkish Journal of Medical Sciences
Background/aim: To evaluate the influence of caffeic acid phenethyl ester (CAPE) on cigarette smoke (CS)-induced cell damage, oxidative stress, and inflammation in human alveolar epithelial cells. Materials and methods: A549 alveolar epithelial cells were divided into control, CS exposure, CAPE, and CS+CAPE treatment groups. Undiluted CS-exposed medium (100%) and three dilutions (50%, 25%, and 10%) of CS-exposed media were applied to cultured A549 cells, which were analyzed after 3 h of incubation. Viability was measured by MTT assay, the gene expressions were evaluated by real-time PCR, and spectrophotometric techniques were used for biochemical assessments. Results: While CS exposure markedly reduced …
Prevention Of Methotrexate-Induced Nephrotoxicity By Concomitantadministration Of Garlic Aqueous Extract In Rat, Walaa Ahmed, Amr Zaki, Taghred Nabil
Prevention Of Methotrexate-Induced Nephrotoxicity By Concomitantadministration Of Garlic Aqueous Extract In Rat, Walaa Ahmed, Amr Zaki, Taghred Nabil
Turkish Journal of Medical Sciences
Background/aim: Methotrexate (MTX) has been widely used for treatment of cancer and rheumatoid arthritis, but its use has been limited by its nephrotoxicity. This study was carried out to determine whether garlic exerts a protective effect against MTX-induced nephrotoxicity. Materials and methods: Nephrotoxicity was induced in rats after a single i.p. injection of MTX (20 mg/kg). Garlic extract (1 mL/100 g b.w.) was given orally for 7 days before and after MTX administration. Serum samples were collected to evaluate urea, creatinine, sodium, phosphorous, potassium, and calcium. Reduced glutathione, catalase, adenosine deaminase, nitric oxide, and malondialdehyde were measured in renal tissue. …
Beraprost Sodium, A Prostacyclin (Pgi) Analogue, Ameliorateslipopolysaccharide-Induced Cellular Injury In Lung Alveolar Epithelial Cells, Si̇nan Vicil, Suat Erdogan
Beraprost Sodium, A Prostacyclin (Pgi) Analogue, Ameliorateslipopolysaccharide-Induced Cellular Injury In Lung Alveolar Epithelial Cells, Si̇nan Vicil, Suat Erdogan
Turkish Journal of Medical Sciences
Human alveolar epithelial cells play a critical role in the pathogenesis of lung diseases. The objective of this study is to determine the contribution of beraprost sodium, a prostaglandin I_2 (PGI_2) analogue, to inflammatory and oxidative events in response to lipopolysaccharide (LPS) in airway epithelial cells. Materials and methods: Human pulmonary alveolar epithelial cells (A549) were pretreated with 10 µM beraprost sodium 30 min before stimulation with 1 µg/mL LPS for 24 h. The cellular viability assessments were evaluated by quantitative MTT test. Catalase activity and glutathione and lipid peroxidation levels were determined using spectrophotometric techniques. mRNA expression analyses were …
Salivary Glucose And Antioxidant Defense Markers In Type Ii Diabetes Mellitus, Sayeeda Mussavira, Mala Dharmalingam, Bindhu Omana Sukumaran
Salivary Glucose And Antioxidant Defense Markers In Type Ii Diabetes Mellitus, Sayeeda Mussavira, Mala Dharmalingam, Bindhu Omana Sukumaran
Turkish Journal of Medical Sciences
To evaluate salivary antioxidant defense markers, their correlation with salivary glucose, and glycemic status in type II diabetes mellitus (DM). Materials and methods: The study included 53 diabetic patients and 40 healthy subjects. Salivary glucose, blood glucose, and uric acid (UA) were determined by specific enzymatic methods. Total antioxidant activity (AOA), glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), and total protein were determined spectrophotometrically. Results: Salivary UA (3.12 vs. 1.89 mg/dL), GSH (47 vs. 11.92 µg/mL), and total protein (375.12 vs. 202.23 mg/dL) were significantly higher (P < 0.001; r = 0.455, 0.735, 0.498 respectively) and AOA (653.1 vs. 897.3 µmol/L) was significantly lower in the DM group (P < 0.001, r = -0.431) compared to healthy controls. Among the antioxidant enzymes, CAT was significantly lower (1214 vs. 9468.9 kat) in the DM group (P < 0.001, r = -0.886). Spearman correlation analyses within the diabetic group showed a strong positive association between salivary glucose and blood glucose (P < 0.001, r = 0.9), salivary glucose and GSH, and salivary glucose and UA. Salivary glucose showed a negative correlation with AOA and CAT (P = 0.008, r = -0.447) in the diabetic group. Conclusion: Findings of this study, showing a strong correlation between salivary glucose and blood glucose as well as changes in antioxidant components in the DM group, suggest that saliva can be used for the diagnosis and management of DM.
The Protective Effect Of Syringic Acid On Ischemia Injury In Rat Brain, Mustafa Güven, Adem Bozkurt Aras, Naci̇ Topaloğlu, Adi̇le Özkan, Hali̇l Murat Şen, Yildiray Kalkan, Ali̇ Okuyucu, Ayla Akbal, Ferhat Gökmen, Murat Çoşar
The Protective Effect Of Syringic Acid On Ischemia Injury In Rat Brain, Mustafa Güven, Adem Bozkurt Aras, Naci̇ Topaloğlu, Adi̇le Özkan, Hali̇l Murat Şen, Yildiray Kalkan, Ali̇ Okuyucu, Ayla Akbal, Ferhat Gökmen, Murat Çoşar
Turkish Journal of Medical Sciences
Brain ischemia and treatment are important topics in neurological science. Free oxygen radicals and inflammation formed after ischemia are accepted as the most significant causes of damage. Currently there are studies on many chemopreventive agents to prevent cerebral ischemia damage. Our aim is to research the preventive effect of the active ingredient in syringic acid, previously unstudied, on oxidative damage in cerebral ischemia. Materials and methods: The rats were randomly divided into 4 groups: control group (no medication or surgical procedure), sham group (artery occlusion), artery occlusion + syringic acid group sacrificed at 6 h, and artery occlusion + syringic …
Vitamin E Modulates Lung Oxidative Stress, Serum Copper, Zinc, And Iron Levels In Rats With Pulmonary Contusion, Mehmet Sirmali, Okan Solak, Tali̇p Çevi̇k, Rana Sirmali, Bünyami̇n Özaydin, Zeynep Gi̇ni̇ş, Yetki̇n Ağaçkiran, Namik Deli̇baş
Vitamin E Modulates Lung Oxidative Stress, Serum Copper, Zinc, And Iron Levels In Rats With Pulmonary Contusion, Mehmet Sirmali, Okan Solak, Tali̇p Çevi̇k, Rana Sirmali, Bünyami̇n Özaydin, Zeynep Gi̇ni̇ş, Yetki̇n Ağaçkiran, Namik Deli̇baş
Turkish Journal of Medical Sciences
To evaluate the effects of oxidant/antioxidant mechanisms and levels of trace elements on trauma-stimulated moderate pulmonary contusions after vitamin E administration. Materials and methods: Sixty-three male Sprague Dawley rats were used. Animals were studied in 4 groups. Vitamin E (150 mg/kg) was injected intraperitoneally 30 min after trauma and on the first and second days. Blood samples were obtained for nitric oxide (NO) levels and superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities. Zinc (Zn^{+2}), copper (Cu^{+2}), and iron (Fe^{+3}) were measured in serum. Results: Lung contusion increased serum and tissue NO levels and SOD activities and decreased GSH-Px activities …
Hepatoprotective And Antioxidant Effects Of Lycopene In Acute Cholestasis, Mehmet Tokaç, Sevtap Aydin, Gökçe Taner, Alper Bi̇lal Özkardeş, Mi̇ne Yavuz Taşlipinar, Mehmet Doğan, Hali̇t Zi̇ya Dündar, Mehmet Kiliç, Ari̇f Ahmet Başaran, Ayşe Nurşen Başaran
Hepatoprotective And Antioxidant Effects Of Lycopene In Acute Cholestasis, Mehmet Tokaç, Sevtap Aydin, Gökçe Taner, Alper Bi̇lal Özkardeş, Mi̇ne Yavuz Taşlipinar, Mehmet Doğan, Hali̇t Zi̇ya Dündar, Mehmet Kiliç, Ari̇f Ahmet Başaran, Ayşe Nurşen Başaran
Turkish Journal of Medical Sciences
Background/aim: Lycopene, which is suggested to be a potent antioxidant, may play a protective role in diseases related to oxidative stress. In order to understand the effects of lycopene in the pathogenesis of cholestasis, we investigated the effects of lycopene on oxidative stress parameters and DNA damage induced by experimental biliary obstruction in the liver tissues and the lymphocytes of Wistar albino rats. Materials and methods: The animals were randomized into 3 groups. The sham group was subjected to a sham operation, the BDL group was subjected to bile duct ligation (BDL), and the BDL+L group was subjected to BDL …
The Relation Between Oxidative Stress Parameters, Ischemic Stroke,And Hemorrhagic Stroke, Ferhat İçme, Özcan Erel, Akkan Avci̇, Sali̇m Satar, Müge Gülen, Selen Acehan
The Relation Between Oxidative Stress Parameters, Ischemic Stroke,And Hemorrhagic Stroke, Ferhat İçme, Özcan Erel, Akkan Avci̇, Sali̇m Satar, Müge Gülen, Selen Acehan
Turkish Journal of Medical Sciences
Background/aim: The aims of this study were to investigate the significance of oxidative stress parameters in the pathogenesis of ischemic stroke and hemorrhagic stroke and to investigate their effects on stroke severity using the National Institutes of Health Stroke Scale (NIHSS). Materials and methods: A total of 92 patients, including 74 with ischemic stroke and 18 with hemorrhagic stroke, and 75 volunteers were enrolled in the study. Total oxidant status (TOS), total antioxidant status (TAS), paraoxonase, stimulating paraoxonase, arylesterase, and thiol levels were measured in both the patient and volunteer groups. NIHSS and oxidative stress index (OSI) scores were calculated. …
Plasma 8-Isopgf2? And Serum Melatonin Levels In Patients With Minimal Cognitive Impairment And Alzheimer Disease, Fevzi̇ye Burcu Şi̇ri̇n, Duygu Kumbul Doğuç, Hüseyi̇n Vural, İbrahi̇m Eren, İkbal İnanli, Recep Sütcü, Namik Deli̇baş
Plasma 8-Isopgf2? And Serum Melatonin Levels In Patients With Minimal Cognitive Impairment And Alzheimer Disease, Fevzi̇ye Burcu Şi̇ri̇n, Duygu Kumbul Doğuç, Hüseyi̇n Vural, İbrahi̇m Eren, İkbal İnanli, Recep Sütcü, Namik Deli̇baş
Turkish Journal of Medical Sciences
Background/aim: F2α-isoprostane is accepted as an oxidative stress indicator and melatonin shows neuroprotective effects by antioxidative and antiamyloidogenic influences. By measuring these in patients diagnosed with minimal cognitive impairment (MCI) and Alzheimer-type dementia, we intended to demonstrate whether the measurement of these markers contributes to early diagnosis of Alzheimer disease (AD) in the MCI stage or not. Materials and methods: Three groups (n = 63) were created: the AD group, MCI group, and control group. Serum melatonin levels were measured by radioimmunoassay method, and plasma total 8-isoPGF2α levels were measured by enzyme immunoassay method. Results: Significant differences were observed in …
Effect Of Capparis Spinosa L. On Cognitive Impairment Induced By D-Galactosein Mice Via Inhibition Of Oxidative Stress, Nergi̇z Hacer Turgut, Haki̇ Kara, Emre Arslanbaş, Derya Güli̇z Mert, Bektaş Tepe, Hüseyi̇n Güngör
Effect Of Capparis Spinosa L. On Cognitive Impairment Induced By D-Galactosein Mice Via Inhibition Of Oxidative Stress, Nergi̇z Hacer Turgut, Haki̇ Kara, Emre Arslanbaş, Derya Güli̇z Mert, Bektaş Tepe, Hüseyi̇n Güngör
Turkish Journal of Medical Sciences
Background/aim: To determine the phenolic acid levels and DNA damage protection potential of Capparis spinosa L. seed extract and to investigate the effect of the extract on cognitive impairment and oxidative stress in an Alzheimer disease mice model. Materials and methods: Thirty BALB/c mice divided into 5 groups (control??, D-galactose, D-galactose + C. spinosa 50, D-galactose + C. spinosa 100, D-galactose + C. spinosa 200) were used. Mice were administered an injection of D-galactose (100 mg/kg, subcutaneous) and orally administered C. spinosa (50, 100, or 200 mg/kg) daily for 8 weeks. Results: Syringic acid was detected and the total amount …
The Effects Of Atorvastatin On Antioxidant/Antiinflammatory Propertiesof Hdls In Hypercholesterolemics, Gülşen Akalin Çi̇ftçi̇, İpek Ertorun, Aysen Akalin, İbrahi̇m Özkan Alataş, Ahmet Musmul
The Effects Of Atorvastatin On Antioxidant/Antiinflammatory Propertiesof Hdls In Hypercholesterolemics, Gülşen Akalin Çi̇ftçi̇, İpek Ertorun, Aysen Akalin, İbrahi̇m Özkan Alataş, Ahmet Musmul
Turkish Journal of Medical Sciences
Background/aim: Hypercholesterolemia is characterized by changes in lipid profile, nitric oxide pathway, and oxidative stress markers, but functions of high-density lipoprotein (HDL) were not well established in hypercholesterolemic subjects treated with atorvastatin. In this study, we aimed to evaluate effects of atorvastatin treatment on functionality of HDL, oxidative stress, and endothelial functions in hypercholesterolemic subjects. Materials and methods: Thirty patients (20 females, 10 males) aged from 40 to 60 years and diagnosed as hypercholesterolemic were included. Patients were treated with 10 mg/day atorvastatin for 3 months. Markers of endothelial functions, namely asymmetric dimethylarginine (ADMA), homocysteine, and nitric oxide (NO), and …
Effects Of Grape Seed Extract On Oxidative Stress And Antioxidant Defensemarkers In Streptozotocin-Induced Diabetic Rats, Muaz Belvi̇ranli, Hakki Gökbel, Ni̇lsel Okudan, Sadik Büyükbaş
Effects Of Grape Seed Extract On Oxidative Stress And Antioxidant Defensemarkers In Streptozotocin-Induced Diabetic Rats, Muaz Belvi̇ranli, Hakki Gökbel, Ni̇lsel Okudan, Sadik Büyükbaş
Turkish Journal of Medical Sciences
Background/aim: To evaluate the effects of grape seed extract (GSE) supplementation on oxidative stress and antioxidant markers in streptozotocin (STZ)-induced diabetic rats. Materials and methods: Thirty-six male rats were divided into the following four groups: control, GSE-supplemented control, diabetic, and GSE-supplemented diabetic. Beginning on day 7 after STZ injection, the rats were administered GSE (100 mg kg-1 day-1) in drinking water for 6 weeks. At the end of week 6, rats were sacrificed by cardiac puncture. Plasma nitric oxide (NO) levels and xanthine oxidase (XO), adenosine deaminase (ADA), and glutathione peroxidase (GPx) activities were analyzed. Results: Both XO and ADA …
Evaluation Of Oxidant And Antioxidant Status In Patients With Vitamin B12 Deficiency, Teki̇n Güney, Murat Alişik, Sema Akinci, Sali̇m Neşeli̇oğlu, İmdat Di̇lek, Özcan Erel
Evaluation Of Oxidant And Antioxidant Status In Patients With Vitamin B12 Deficiency, Teki̇n Güney, Murat Alişik, Sema Akinci, Sali̇m Neşeli̇oğlu, İmdat Di̇lek, Özcan Erel
Turkish Journal of Medical Sciences
Background/aim: The aim of this study was to examine the theory that oxidative stress might have an important mediating effect on the deleterious results of vitamin B12 metabolism deficiency seen throughout the body. Materials and methods: Forty patients with vitamin B12 deficiency and 40 healthy controls were included in the study. Venous blood samples were collected from all participants to evaluate serum vitamin B12, homocysteine, methylmalonic acid, total antioxidant status (TAS), and total oxidant status (TOS) levels in the pre- and posttreatment periods. Results: There were no significant differences in TAS, TOS, or oxidative stress index (OSI) levels between the …
Parp Inhibition Prevents Acetaminophen-Induced Liver Injury And Increases Survival Rate In Rats, Melahat Dönmez, Bülent Uysal, Yavuz Poyrazoğlu, Yeşi̇m Er Öztaş, Türker Türker, Ümi̇t Kaldirim, Ahmet Korkmaz
Parp Inhibition Prevents Acetaminophen-Induced Liver Injury And Increases Survival Rate In Rats, Melahat Dönmez, Bülent Uysal, Yavuz Poyrazoğlu, Yeşi̇m Er Öztaş, Türker Türker, Ümi̇t Kaldirim, Ahmet Korkmaz
Turkish Journal of Medical Sciences
Acetaminophen (APAP) overdose results in severe liver damage that may develop into acute liver failure. Recent studies have demonstrated that inhibition of poly(ADP-ribose) polymerase (PARP) decreases tissue necrosis and inflammation. We evaluated the efficacy of 3-aminobenzamide (3-AB), a PARP inhibitor, in a rodent model of APAP-induced hepatotoxicity. Materials and methods: Twenty-four Sprague-Dawley rats were divided equally into 3 experimental groups: sham group, APAP group, and APAP + 3-AB group. In the experimental treatment groups APAP was administered orally at 1 g/kg and, in the APAP + 3-AB group, 3-AB was administered intraperitoneally at a dose of 20 mg/kg exactly 1 …
Protective Effects Of Erdosteine, Vitamin E, And Vitamin C On Renal Injury Induced By The Ischemia-Reperfusion Of The Hind Limbs In Rats, Rana Sirmali, Abdullah Armağan, Faruk Öktem, Efkan Uz, Aynur Kirbaş, Soner Dönmez, Haci Ramazan Yilmaz, Mesrur Selçuk Silay, Mehmet Sirmali
Protective Effects Of Erdosteine, Vitamin E, And Vitamin C On Renal Injury Induced By The Ischemia-Reperfusion Of The Hind Limbs In Rats, Rana Sirmali, Abdullah Armağan, Faruk Öktem, Efkan Uz, Aynur Kirbaş, Soner Dönmez, Haci Ramazan Yilmaz, Mesrur Selçuk Silay, Mehmet Sirmali
Turkish Journal of Medical Sciences
To compare the protective efficacy of erdosteine and vitamins C and E against renal injury caused by hind limb ischemia-reperfusion (I/R). Materials and methods: Rats were split into 4 groups: group I as the control, group II as I/R, group III as I/R + erdosteine, and group IV as I/R + vitamins C and E. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities and malondialdehyde (MDA) tissue levels were determined. Results: MDA levels were found comparable with the control group in groups II and III. However, they were considerably decreased in group IV when compared to group II …
Effects Of Melatonin And Theanine Administration On Pentylenetetrazole-Inducedseizures And Brain Tissue Oxidative Damage In Ovariectomized Rats, Shadi Choopankareh, Farzaneh Vafaee, Mohammad Naser Shafei, Hamid Reza Sadeghnia, Reza Salarinia, Leila Zarepoor, Mahmoud Hosseini
Effects Of Melatonin And Theanine Administration On Pentylenetetrazole-Inducedseizures And Brain Tissue Oxidative Damage In Ovariectomized Rats, Shadi Choopankareh, Farzaneh Vafaee, Mohammad Naser Shafei, Hamid Reza Sadeghnia, Reza Salarinia, Leila Zarepoor, Mahmoud Hosseini
Turkish Journal of Medical Sciences
Background/aim: The effects of coadministration of melatonin and theanine (Mel/Thea) on pentylenetetrazole (PTZ)-induced seizures and brain tissue oxidative damage were investigated in ovariectomized (OVX) and sham-operated rats. Materials and methods: The rats were divided into the following groups: 1) sham, 2) ovariectomized (OVX), 3) sham-PTZ, 4) OVX-PTZ, 5) sham-Mel/Thea-PTZ, and 6) OVX-Mel/Thea-PTZ. Groups 1-4 received saline, while groups 5 and 6 received a combination of Mel/Thea for 6 weeks. All animals except for those in groups 1 and 2 received a single injection of PTZ. Results: The OVX-PTZ group had higher generalized tonic-clonic seizure (GTCS) latency compared to the sham-PTZ …