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Comparative Study On Six Different Long Term Commercial Extenders For Fresh Boar Semen, Kampon Kaeoket, Titisa Srisowanna, Ubonrat Wichaidit, Panida Chanapiwat, Sukanya Manee-In
Comparative Study On Six Different Long Term Commercial Extenders For Fresh Boar Semen, Kampon Kaeoket, Titisa Srisowanna, Ubonrat Wichaidit, Panida Chanapiwat, Sukanya Manee-In
The Thai Journal of Veterinary Medicine
In Thailand, there are numerous commercial extenders for preservation of boar semen; however, no study has been carried out to compare these extenders in view of maintaining of sperm motility, plasma membrane integrity and acrosome integrity during cold storage. The aim of this study was to assess the percentage of sperm motility, HOST positive, viability and acrosome integrity of boar spermatozoa extended in Merck-III, Androstar®Plus, ModenaTM, NUTRIXcell®, VITASEM LD, Duragen and Dofu goldTM. Ejaculated from boars (n=6, one ejaculate from each boar) were collected and sub-samples (splited samples) were diluted (3x109 spermatozoa/100 ml) in the different extenders and stored for …
The Use Of Computer-Assisted Sperm Analysis For Discriminating Series Of Motility Pattern Of Frozen-Thawed Boar Semen, Chanyuth Tretipskul, Kakanang Buranaamnuay, Seri Koonjaenak, Padet Tummaruk, Mongkol Techakumphu
The Use Of Computer-Assisted Sperm Analysis For Discriminating Series Of Motility Pattern Of Frozen-Thawed Boar Semen, Chanyuth Tretipskul, Kakanang Buranaamnuay, Seri Koonjaenak, Padet Tummaruk, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
Computer-assisted sperm analysis (CASA) was initiated to reduce subjective bias on the motility assessment and to discriminate a series of motility patterns of boar semen. The objectives of this present study were to evaluate the motility pattern of frozen-thawed boar semen at 0 and 60 min after thawing using CASA. Forty one ejaculates from Landrace (n=14), Yorkshire (n=12) and Duroc (n=15) boars were cryopreserved and included in the experiment. The semen was thawed at 37°C for 30 sec. The post-thawed sperm qualities including subjective motility, plasma membrane integrity and motility pattern were determined immediately after thawing (T0) and at 60 …