Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Medicine and Health Sciences
Effect Of Dna Base Modification On Polymerase Chain Reaction Efficiency And Fidelity, Jan A. Sikorsky
Effect Of Dna Base Modification On Polymerase Chain Reaction Efficiency And Fidelity, Jan A. Sikorsky
Theses, Dissertations and Capstones
Polymerase stop assays, used to quantify DNA damage, assume single lesions are sufficient to block thermostable DNA polymerase progression. To explore this assumption, 90 base oligonucleotides containing normal or modified DNA bases were amplified using real-time PCR. Data implied that the PCR efficiency was influenced to differing degrees depending on which base lesion was present on the input oligonucleotide; specifically, while reactions with templates containing a single 8-oxo-7,8-dihydro-2í-deoxyguanosine (8-oxodG) were not noticeably altered, the presence of a single 8-oxo-7,8-dihydro-2í-deoxyadenosine, an abasic site, or a cis-syn thymidine dimer (TT dimer) dramatically delayed amplification. In addition, the presence of two tandem 8-oxodGs …
Mechanisms Of Growth Hormone Enhancement Of Excitatory Synaptic Transmission In Hippocampus, Ghada Saad Zaglool Ahmed Mahmoud
Mechanisms Of Growth Hormone Enhancement Of Excitatory Synaptic Transmission In Hippocampus, Ghada Saad Zaglool Ahmed Mahmoud
Theses, Dissertations and Capstones
Growth hormone (GH) deficiency is associated with impaired learning and memory. One possible target for GH effects on memory is the hippocampus, a brain region containing GH receptors (GHRs). To determine if GH acutely alters hippocampal function, recombinant human GH (rhGH) was applied to in vitro rat hippocampal brain slices. Extracellular recordings were used to assess effects of GH on the field EPSP (fEPSP) and long-term potentiation (LTP) of the fEPSP. GHR expression was measured in GH-treated and control rat hippocampal slices using RT-PCR. The GH signaling pathway was investigated by studying the effect of GH on the fEPSP after …