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Full-Text Articles in Medicine and Health Sciences
An Immunolohistochemical Study Of Anti-Apoptotic Bcl-2 In The Pig’S Oviduct During The Oestrous Cycle, Paisan Tienthai
An Immunolohistochemical Study Of Anti-Apoptotic Bcl-2 In The Pig’S Oviduct During The Oestrous Cycle, Paisan Tienthai
The Thai Journal of Veterinary Medicine
The purpose of this study was to determine the localization of Bcl-2 protein in porcine oviducts. Anti-human Bcl-2 monoclonal antibody was used to localize Bcl-2 protein immunohistochemically in the uterotubal junction (UTJ), isthmus and ampulla of pre-ovulatory (n=10) and post-ovulatory (n=10) sows. The Bcl-2 protein was detected in quantity in the intracytoplasm of the epithelial cells, some types of leukocytes in the epithelial layer and in the subepithelial connective tissue, in fibroblasts, as well as smooth muscle cells of the UTJ, isthmus and ampulla in the pre-ovulatory period. In contrast, the expression of Bcl-2 was lower in the oviduct tissues …
Intrauterine Artificial Insemination In The Pig: The Techniques, The Applications And The Limitations, Tuempong Wongtawan, Peerapong Samransarp, Padet Tummaruk
Intrauterine Artificial Insemination In The Pig: The Techniques, The Applications And The Limitations, Tuempong Wongtawan, Peerapong Samransarp, Padet Tummaruk
The Thai Journal of Veterinary Medicine
This review aims to update information on the success of intra-uterine and deep intra-uterine insemination in the pig. Three artificial insemination (AI) techniques in pigs included conventional AI (semen is deposited intra cervically), intra-uterine insemination (IUI) and deep intra-uterine insemination (DIUI). With conventional AI, it has been shown that more than 90% of spermatozoa are lose before reaching the oviducts. Therefore, new techniques for AI were developed in order to maximize the use of boar semen and to reduce semen backflow, which can reduce pregnancy rates. The advantages of intra-uterine and deep intra-uterine insemination include a reduction in the number …
Hyaluronan In The Sow’S Oviduct: Its Effect On Boar Sperm Morphology And Function, Paisan Tienthai
Hyaluronan In The Sow’S Oviduct: Its Effect On Boar Sperm Morphology And Function, Paisan Tienthai
The Thai Journal of Veterinary Medicine
During sperm transport in the female pig, a proportion of the spermatozoa is arrested at the uterotubal junction and the isthmus, where an oviductal sperm reservoir is built up. The sperm reservoir maintains sperm viability and controls the release of fertilizing spermatozoa so that only a small subpopulation reaches the site of fertilization, thus diminishing the risk of polyspermy. In vitro research has focused on sperm binding as the main mechanism of sperm storage, sperm release and modulation of capacitation, but little attention has been paid to the sperm reservoir fluid, its composition and the control of capacitation in vivo. …
Fas Ligand Expression In The Porcine Oviduct, Paisan Tienthai, Yumi Hoshino
Fas Ligand Expression In The Porcine Oviduct, Paisan Tienthai, Yumi Hoshino
The Thai Journal of Veterinary Medicine
The expression of Fas/Fas Ligand (FasL) is thought to be involved in the immune privileged status of the testis, cornea and the placenta. The objective of this study was to see whether FasL mRNA was expressed in the epithelium of the porcine oviduct, where spermatozoa, oocytes and developing embryos avoid elimination by the female immune system during the estrous cycle. The distribution of Fas/FasL might provide information about immune privileged sites in the pig oviduct. The gene expression of FasL was detected in the uterotubal junction (UTJ), isthmus and ampulla of the pig oviductal epithelium, during both pre- and post-ovulatory …