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Full-Text Articles in Medicine and Health Sciences
Characterization Of Seca-Sod Operon In Borrellia Burgdorferi., Tonya Lynn Nichols
Characterization Of Seca-Sod Operon In Borrellia Burgdorferi., Tonya Lynn Nichols
Electronic Theses and Dissertations
Borrelia burgdorferi, the causative agent of Lyme disease, has been characterized as a microaerophilic spirochete. O2 consumption and utilization potentially yield reactive oxygen intermediates, such as superoxide, hydroxyl radicals, and hydrogen peroxide. This study investigated the expression of the sod gene, which encodes the only, identified oxidative defense mechanism in B. burgdorferi. Using primer extension analysis and RT-PCR, it was found that sod and secA are organized as a single transcriptional unit under the control of σ70-like promoter upstream of the secA open reading frame. Generally, gene expression decreases with increased distance from the promoter; however, secA expression was observed …
Molecular Studies Involving The Rev Protein Of Caprine Arthritis Encephalitis Virus And Visna Virus., Bridget Michele Graves
Molecular Studies Involving The Rev Protein Of Caprine Arthritis Encephalitis Virus And Visna Virus., Bridget Michele Graves
Electronic Theses and Dissertations
Caprine Arthirtis Encephalitis Virus (CAEV) and Visna Virus are two viruses of the lentivirus family. They encode three structural genes (gag, pol, and env) and two regulatory genes (rev and tat). The Rev protein regulates Gag, Pol and Env expression by transporting their mRNAs to the cytoplasm by binding to the RRE (Rev Response Element) found on their mRNAs. Previous studies have indicated that Rev may be toxic to transfected cells, overexpression of exogenous RREs or a better binding RRE can inhibit Rev activity and Rev-C (CAEV Rev) can trans-activate RRE-V (Visna Virus …
Behavioral Changes In Adult C57bl/6j Mice Following Prenatal Exposure To Ethanol., Kevin Wade Nunley
Behavioral Changes In Adult C57bl/6j Mice Following Prenatal Exposure To Ethanol., Kevin Wade Nunley
Electronic Theses and Dissertations
Fetal Alcohol Syndrome (FAS) labels children with physical, mental and behavioral deficits exposed to alcohol in utero. Current research indicates that timing of alcohol exposure of the embryo/fetus is a critical determinant of the behavioral deficits associated with FAS. This study represents a model for binge drinking, in which C57BL mouse embryos were exposed to alcohol during 2 separate critical periods of brain development, gestational day (GD) 7 or 8. As adults, the offspring were tested to determine if loco-motor activity and emotional reaction to a novel environment had been affected. Significant differences due to treatment and sex were …