Medicine and Health Sciences Commons^™

Coronavirus-Like Particles In Porcine Kupffer Cell Culture, Roongroje Thanawongnuwech, Jacqueline K. Kinyamu, Jean A. Olsen

The Thai Journal of Veterinary Medicine

Coronavirus-like particles were observed by transmission electron microscopy in smooth-walled vesicles of Golgi, or endoplasmic reticulum (ER) in a primary Kupffer cell culture. The culture came from a clinically normal 10-day-old pig, from a swine herd that was known to be infected with porcine respiratory coronavirus (PRCV) and porcine reproductive and respiratory syndrome virus (PRRSV). Budding profiles of virions were observed in the smooth-walled vesicles and later, apparently free viruses accumulated in the collecting vesicles. The virions were enveloped and pleomorphic, with a diameter of 50-100 nm. The virus particles contained an external envelope and an inner membrane and a …