Medicine and Health Sciences Commons^™

Hybrid Vector And Method Resulting In Protein Overproduction By Eukaryotic Cells, Robert E. Rhoads, Arrico De Benedetti

Molecular and Cellular Biochemistry Faculty Patents

A hybrid vector carrying a first and second DNA segments operationally linked thereto, the first DNA segment encoding a protein capable of cross-linking to the cap structure of mRNA and mediating ribosome-binding, and the second DNA segment encoding a polypeptide or protein, the vector being capable of replication, transcription and translation to express the factor and the polypeptide or protein upon transformation of a eukaryotic host, and the polypeptide or protein being expressed at a level higher than the level of expression thereof in the absence of the first DNA segment. A eukaryotic host is transformed with this hybrid vector. …