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Enhancement Of Human Immunodeficiency Virus Type 1 Infection By Cationic Liposomes: The Role Of Cd4, Serum And Liposome-Cell Interactions, Krystyna Konopka, L. L. Stamatatos, C. E. Larsen, B. R. Davis, Nejat Düzgüneş

All Dugoni School of Dentistry Faculty Articles

We have reported previously the enhancement of the infectivity of human immunodeficiency virus type 1 (HIV- 1) by liposomes composed of the cationic lipid N[2,3-(dioleyloxy) propyl]-N,N,N-trimethylammonium chloride (DOTMA). To determine the mechanism by which this process occurs, we have investigated the role of CD4, serum concentration and liposome-cell interactions in the DOTMA-mediated stimulation of HIV-1 infection of A3.01 cells. Serum alone significantly inhibited the binding and infectivity of HIV-1, but DOTMA-mediated enhancement of infectivity was more pronounced in the presence of serum than in its absence. HIV-1 binding to cells was increased in the presence of DOTMA liposomes, DEAE-dextran and …