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Full-Text Articles in Medicine and Health Sciences
Rna-Dependent Selenocysteine Biosynthesis In Eukaryotes And Archaea, Sotiria Palioura
Rna-Dependent Selenocysteine Biosynthesis In Eukaryotes And Archaea, Sotiria Palioura
Yale Medicine Thesis Digital Library
Selenocysteine (Sec), the 21st genetically encoded amino acid, is the major metabolite of the micronutrient selenium. Sec is inserted into nascent proteins in response to a UGA codon. The substrate for ribosomal protein synthesis is selenocysteinyl-tRNASec. While the formation of Sec-tRNASec from seryl-tRNASec by a single bacterial enzyme selenocysteine synthase (SelA) has been well described, the mechanism of Sec-tRNASec formation in archaea and eukaryotes remained poorly understood. Herein, biochemical and genetic data provide evidence that, in contrast to bacteria, eukaryotes and archaea utilize a different route to Sec-tRNASec that requires the tRNASec-dependent conversion of O-phosphoserine (Sep) to Sec. In this …
Nmr Studies Of Acyl Chain-Protein Interactions, Mark Christian Oswood
Nmr Studies Of Acyl Chain-Protein Interactions, Mark Christian Oswood
Yale Medicine Thesis Digital Library
An approach employing heteronuclear NMR is presented for monitoring the interactions between long-chain fatty acids and proteins which are involved in their synthesis and intracellular transport. Two naturally occurring fatty acids, hexadecanoic (palmitic) and cis-9-10-methylene-hexadecanoic (MHA) acids were labeled using a biosynthetic scheme with 13C acetate as a source. The biosynthetic incorporation of 13C from a two carbon source resulted in sequential pairs of 13C atoms in the product. When combined with double quantum spectroscopy, a highly efficient suppression of natural abundance background resonances results. Proton-detected two and three-dimensional experiments which correlate double quantum carbon chemical shifts with proton shifts …
Chemiosmotic Properties Of Isolated Secretory Granules From Parotid Glands Of Normal And Isoproterenol Treated Rats, Peter Raymond Arvan
Chemiosmotic Properties Of Isolated Secretory Granules From Parotid Glands Of Normal And Isoproterenol Treated Rats, Peter Raymond Arvan
Yale Medicine Thesis Digital Library
Secretory granule fractions were prepared isoosmotically from the parotid glands of normal rats and from rats that had received repeated injections of isoproterenol. These granules, purified extensively, are stable after isolation, making it possible to study biophysical parameters which may relate to their role in intracellular transport and secretion.Normal parotid granules have an internal pH of (TURN)6.8 and a low ionic permeability with respect to internal buffering capacity. By contrast, granules from the treated rats have an internal pH ranging from (TURN)7.7 in (0.3 M) sucrose media to (TURN)7.1 in (100 mM) KCl media; suggesting a large ionic permeability with …
Studies Of Plasma Membrane Domain-Specific Molecules In Isolated And Cultured Rat Hepatocytes, Pamela Leslie Zeitlin
Studies Of Plasma Membrane Domain-Specific Molecules In Isolated And Cultured Rat Hepatocytes, Pamela Leslie Zeitlin
Yale Medicine Thesis Digital Library
Single rat hepatocytes, prepared by collagenase perfusion of liver, lost the three morphologically and functionally distinct plasma membrane domains that exist in situ (sinusoidal, bile canalicular, and lateral). Hepatocytes placed in monolayer culture re-established morphologically distinct plasma membrane regions analogous to the original three domains. The behavior of several domain-specific markers was studied in these cells. The asialoglycoprotein (ASGP) receptor, an integral membrane glycoprotein normally restricted at the surface to the sinusoidal domain in situ, was retained in both single hepatocytes (> 95%) and in cells after 24 hr in monolayer culture but at lower levels. Cytochemistry with asialo-oroso-mucoid-horseradish peroxidase …
Neutron Diffraction Analysis Of The Structure Of Rod Photoreceptor Membranes In Intact Retinas, Mark Jay Yeager
Neutron Diffraction Analysis Of The Structure Of Rod Photoreceptor Membranes In Intact Retinas, Mark Jay Yeager
Yale Medicine Thesis Digital Library
No abstract provided.
Limited Fragmentation Of Specifically Labeled Rhodopsins, Jordan S. Pober
Limited Fragmentation Of Specifically Labeled Rhodopsins, Jordan S. Pober
Yale Medicine Thesis Digital Library
No abstract provided.
The Effects Of Hypothalamic And Reticular Stimulation On Evoked Responses In The Visual System Of The Cat, Carl Chungming Chi
The Effects Of Hypothalamic And Reticular Stimulation On Evoked Responses In The Visual System Of The Cat, Carl Chungming Chi
Yale Medicine Thesis Digital Library
No abstract provided.