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Articles 1 - 30 of 35
Full-Text Articles in Medicine and Health Sciences
Ua66/14/4 Kentucky Partnership Newsletter, Vol. Ii, No. 9, Kentucky Partnership For Farm Family Health & Safety
Ua66/14/4 Kentucky Partnership Newsletter, Vol. Ii, No. 9, Kentucky Partnership For Farm Family Health & Safety
University Organizations
Newsletter created by and about the Kentucky Partnership for Farm Family Health & Safety.
Selective Increases In Antibody Isotypes And Immunoglobulin G Subclass Responses To Secreted Antigens In Tuberculosis Patients And Healthy Household Contacts Of The Patients, R Hussain, G Dawood, N Abrar, Z Toossi, A Minai, M Dojki, J J. Ellner
Selective Increases In Antibody Isotypes And Immunoglobulin G Subclass Responses To Secreted Antigens In Tuberculosis Patients And Healthy Household Contacts Of The Patients, R Hussain, G Dawood, N Abrar, Z Toossi, A Minai, M Dojki, J J. Ellner
Department of Pathology and Laboratory Medicine
Mycobacterium tuberculosis-specific antibodies (immunoglobulin M [IgM], IgE, IgG, and IgG subclasses) were determined in 164 tuberculosis patients (pulmonary involvement, n = 135; lymph node involvement, n = 29), 59 healthy household contacts (HC), and 51 healthy endemic donors (EC) by a quantitative enzyme-linked immunosorbent assay for reactivity with culture filtrate. Among the isotypes, significant differences between tuberculosis patient groups with either pulmonary or lymph node involvement and healthy control groups (HC and EC) were detected only for IgG (P < 0.001) and IgG1 (P < 0.001) antibodies. Pulmonary patients also showed a significant difference with IgM (P < 0.01) and IgE (P < 0.05) antibodies. HC showed elevation of only IgM antibodies compared with EC, indicating that IgM antibodies may be an indicator of recent infection with M. tuberculosis. These results suggest that the switching of IgM antibody response to IgG1 is a critical event in disease progression. Polyclonal IgG1, IgG3, and IgE antibodies also showed significant elevation (P < 0.05) in patients compared with EC. A strong correlation (rho = 0.254; P < 0.003) was observed between M. tuberculosis-specific IgG1 and polyclonal IgG1 in patients, suggesting that activations of antigen-specific and polyclonal antibodies are related events. No correlation was found between IgG1 antibodies and purified protein derivative skin test results. Since IgG1 antibody responses to culture filtrate are present only after disease establishment, IgG1 responses could provide a useful diagnostic marker of disease.
Protecting Your Back: Weight Room Management, Peter J. Titlebaum, Gordon Rackley
Protecting Your Back: Weight Room Management, Peter J. Titlebaum, Gordon Rackley
Health and Sport Science Faculty Publications
Weight room supervision can be a formidable undertaking without the necessary preparations and precautions. Weight room supervisors are much like the Secret Service; that is, no matter what happens, if bad, it is the supervisor's fault. Risk management is more important now than at any point in history. The keys to preventing problems and creating an efficient weight room facility include a highly trained staff and a thoroughly detailed employee manual designed specifically for the weight room.
Involvement Of P59FynT In Interleukin-5 Receptor Signaling, Mark W. Appleby, James D. Kerner, Sylvia Chien, Charles R. Maliszewski, Subbarao Bondada, Roger M. Perlmutter
Involvement Of P59FynT In Interleukin-5 Receptor Signaling, Mark W. Appleby, James D. Kerner, Sylvia Chien, Charles R. Maliszewski, Subbarao Bondada, Roger M. Perlmutter
Microbiology, Immunology, and Molecular Genetics Faculty Publications
Previous studies implicate the nonreceptor protein tyrosine kinase (PTK) p59fyn in the propagation of signals from the B cell antigen receptor. To elucidate the functions of this kinase, we examined B cell responsiveness in mice engineered to lack the hematopoietic isoform of p59fyn. Remarkably, antigen receptor signaling was only modestly defective in fynTnull B cells. In contrast, signaling from the interleukin (IL)-5 receptor which ordinarily provides a comitogenic stimulus with antiimmunoglobulin, was completely blocked. Our results document the importance of p59fynT in IL-5 responses in B cells, and they support a general model …
Transactivation Of The Moloney Murine Leukemia Virus And T-Cell Receptor Beta-Chain Enhancers By Cbf And Ets Requires Intact Binding Sites For Both Proteins., Wanwen Sun, Barbara J. Graves, Nancy A. Speck
Transactivation Of The Moloney Murine Leukemia Virus And T-Cell Receptor Beta-Chain Enhancers By Cbf And Ets Requires Intact Binding Sites For Both Proteins., Wanwen Sun, Barbara J. Graves, Nancy A. Speck
Dartmouth Scholarship
The Moloney murine leukemia virus (Mo-MLV) enhancer contains binding sites (LVb and LVc) for the ets gene family of proteins and a core site that binds the polyomavirus enhancer-binding protein 2/core-binding factor (cbf) family of proteins. The LVb and core sites in the Mo-MLV enhancer contribute to its constitutive activity in T cells. All three binding sites (LVb, LVc, and core) are required for phorbol ester inducibility of the Mo-MLV enhancer. Adjacent binding sites for the ets and cbf proteins likewise constitute a phorbol ester response element within the human T-cell receptor beta-chain (TCR beta) enhancer and contribute to constitutive …
Ph And Calcium Dependence Of Hemolysis Due To Rickettsia Prowazekii: Comparison With Phospholipase Activity, David M. Ojcius, M. Thibon, C. Mounier, Alice Dautry-Varsat
Ph And Calcium Dependence Of Hemolysis Due To Rickettsia Prowazekii: Comparison With Phospholipase Activity, David M. Ojcius, M. Thibon, C. Mounier, Alice Dautry-Varsat
All Dugoni School of Dentistry Faculty Articles
Rickettsia prowazekii invades nucleated cells through phagocytosis and subsequently proliferates in the cytoplasm of the host cell. Hemolysis and a phospholipase A2 (PLA2) activity at neutral pHs have previously been reported; even though the phagosomal environment is most likely acidic. We here show that R. prowazekii and R. typhi also lyse erythrocytes at mildly acidic pHs, compatible with an early phagosomal compartment. For R. prowazekii, hemolysis at an acidic pH but not a neutral pH is enhanced by Ca2+, raising the possibility that more than one membranolytic factor may be produced by the rickettsiae. The rickettsiae alone display PLA2 activity, …
Military Recreation Programs: Their Role In Quality Of Life Of United States' Service Men And Women And Their Families, Michael Gorham
Military Recreation Programs: Their Role In Quality Of Life Of United States' Service Men And Women And Their Families, Michael Gorham
Masters Theses & Specialist Projects
This thesis was designed to determine the importance of military recreation programs in improving the quality of life of United States service men and women. The study utilized a survey conducted on Fort Campbell, Kentucky, in the Spring of 1995. The results were analyzed individually and as a group. The findings were that military recreation programs play an important role in the quality of life of United States service men and women and their families. These findings were supported by the review of related literature and results from the survey which showed that 70% of the respondent's "Agreed" or "Strongly …
A 55 Year Old Diabetic Presenting With A Rash And Polyneuropathy, S Hyder, H Rasool, H Naqvi, Naila Kayani
A 55 Year Old Diabetic Presenting With A Rash And Polyneuropathy, S Hyder, H Rasool, H Naqvi, Naila Kayani
Department of Pathology and Laboratory Medicine
No abstract provided.
A Tef-1-Independent Mechanism For Activation Of The Simian Virus 40 (Sv40) Late Promoter By Mutant Sv40 Large T Antigens., Paul Casaz, Phillip W. Rice, Charles N. Cole, Ulla Hansen
A Tef-1-Independent Mechanism For Activation Of The Simian Virus 40 (Sv40) Late Promoter By Mutant Sv40 Large T Antigens., Paul Casaz, Phillip W. Rice, Charles N. Cole, Ulla Hansen
Dartmouth Scholarship
Simian virus 40 (SV40) large tumor antigen (T antigen) stimulates the activity of the SV40 late promoter and a number of cellular and other viral promoters. We have characterized the ability of T antigens with mutations in the DNA-binding domain and within the N-terminal 85 residues to activate the SV40 late promoter. T antigens lacking both nonspecific and sequence-specific DNA-binding activities were able to induce the late promoter. Mutations within the N-terminal 85 residues of T antigen diminished activation by less than twofold. Activation by wild-type and most of the mutant T antigens required intact binding sites for the cellular …
Heterozygosity Mapping Of Partially Congenic Lines: Mapping Of A Semidominant Neurological Mutation, Wheels ( Whl), On Mouse Chromosome 4, Patrick M. Nolan, Patricia J. Sollars, Barbara A. Bohne, Warren J. Ewens, Gary E. Pickard, Maja Bucan
Heterozygosity Mapping Of Partially Congenic Lines: Mapping Of A Semidominant Neurological Mutation, Wheels ( Whl), On Mouse Chromosome 4, Patrick M. Nolan, Patricia J. Sollars, Barbara A. Bohne, Warren J. Ewens, Gary E. Pickard, Maja Bucan
School of Veterinary and Biomedical Sciences: Faculty Publications
We identified a semidominant, chemically induced, mouse use mutation with a complex array of abnormal behaviors including bidirectional circling and hyperactivity, abnormal circadian rhythmicity and abnormal responses to light. In this report, we genetically and phenotypically characterized the circling/ waltzing component of the abnormal behavior. We mapped the locus controlling this trait by heterozygosity mapping of partially congenic lines carrying the mutagenized chromosome outcrossed to different inbred strains for three generations. Analysis of 68 PCR-based markers in 13 affected individuals indicated that the mutant locus, named Wheels (Whl), resides in the subcentromeric portion of mouse chromosome 4. The …
Transcriptional Activity Of Core Binding Factor-Alpha (Aml1) And Beta Subunits On Murine Leukemia Virus Enhancer Cores., Ari L. Zaiman, Amy F. Lewis, Barbara E. Crute, N. A. Speck, Jack Lenz
Transcriptional Activity Of Core Binding Factor-Alpha (Aml1) And Beta Subunits On Murine Leukemia Virus Enhancer Cores., Ari L. Zaiman, Amy F. Lewis, Barbara E. Crute, N. A. Speck, Jack Lenz
Dartmouth Scholarship
Core binding factor (CBF), also known as polyomavirus enhancer-binding protein 2 and SL3 enhancer factor 1, is a mammalian transcription factor that binds to an element termed the core within the enhancers of the murine leukemia virus family of retroviruses. The core elements of the SL3 virus are important genetic determinants of the ability of this virus to induce T-cell lymphomas and the transcriptional activity of the viral long terminal repeat in T lymphocytes. CBF consists of two subunits, a DNA binding subunit, CBF alpha, and a second subunit, CBF beta, that stimulates the DNA binding activity of CBF alpha. …
Altered Platelet Activating Factor Metabolism In Insulin Dependent Diabetes Mellitus, R A. Memon, S A. Saeed, A Jabbar, A Jafri, A H. Gilani, S Saleem, H Akbani
Altered Platelet Activating Factor Metabolism In Insulin Dependent Diabetes Mellitus, R A. Memon, S A. Saeed, A Jabbar, A Jafri, A H. Gilani, S Saleem, H Akbani
Department of Biological & Biomedical Sciences
Diabetes mellitus is associated with several abnormalities of platelet function. Recent studies have shown that the blood level of platelet activating factor (PAF), a potent inducer of platelet aggregation, is elevated in insulin dependent diabetes mellitus (IDDM) and remains unchanged in non-insulin dependent diabetes mellitus (NIDDM) patients. However, the mechanism of this increase in PAF levels has not been determined. In this study we have measured the activity of plasma PAF acetylhydrolase (an enzyme that regulates PAF levels) and lipoprotein levels in control subjects and diabetic patients. The data presented show that plasma PAF acetylhydrolase activity is significantly decreased in …
Protein-Peptide Interactions Analyzed With The Yeast Two-Hybrid System, Meijia Yang, Zining Wu, Stanley Fields
Protein-Peptide Interactions Analyzed With The Yeast Two-Hybrid System, Meijia Yang, Zining Wu, Stanley Fields
Dartmouth Scholarship
The yeast two-hybrid system was used to screen a library of random peptides fused to a transcriptional activation domain in order to identify peptides capable of binding to the retinoblastoma protein (Rb). Seven peptides were identified, allof which contain the Leu-X-Cys-X-Glu motif found in Rb-binding proteins, although their activity in the yeast assay variedover a 40-fold range. Mutagenesis of the DNA encoding two of these peptides followed by screening in the two-hybrid systemallowed the delineation of residues apart from the invariant Leu, Cys and Glu that affect binding to Rb. Binding affinities of a peptide and one of its variants …
Human Performance Lab Newsletter, Spring 1995, St. Cloud State University
Human Performance Lab Newsletter, Spring 1995, St. Cloud State University
Human Performance Lab Newsletter
Contents of this issue include:
- Kelly's Corner by Jack Kelly
- ACSM Promotes Healthy People 2000 by Bryan Huft
- Publications and Presentations
- Recipes
- Turn off the TV - Turn on the Fitness! by Deb Meierhofer
- Drink Up! by Janice Engebretson
Ua66/14/4 Kentucky Partnership Newsletter, Vol. Ii, No. 8, Kentucky Partnership For Farm Family Health & Safety
Ua66/14/4 Kentucky Partnership Newsletter, Vol. Ii, No. 8, Kentucky Partnership For Farm Family Health & Safety
University Organizations
Newsletter created by and about the Kentucky Partnership for Farm Family Health & Safety.
Restoration Of Circadian Behavior By Anterior Hypothalamic Heterografts, Patricia J. Sollars, Daniel P. Kimble, Gary E. Pickard
Restoration Of Circadian Behavior By Anterior Hypothalamic Heterografts, Patricia J. Sollars, Daniel P. Kimble, Gary E. Pickard
School of Veterinary and Biomedical Sciences: Faculty Publications
The suprachiasmatic nucleus (SCN) of the anterior hypothalamus (AH) is a circadian oscillator and an important component of the mammalian circadian system. To determine whether the SCN is the dominant circadian pacemaker responsible for generating a species-typical characteristic of circadian rhythms [i.e., period length (T)], neural transplantation was conducted using fetal AH donors of different species and SCN-lesioned (SCNx) hosts. The circadian behavior of each of the three donor species is clearly distinguishable by its species-typical T. The extent of SCN pacemaker autonomy was assessed by noting whether the period of the restored circadian rhythm following heterograft transplantation was characteristic …
Dynamic Diseases In Neurology And Psychiatry, John Milton, Deborah Black
Dynamic Diseases In Neurology And Psychiatry, John Milton, Deborah Black
WM Keck Science Faculty Papers
Thirty-two (32) periodic diseases of the nervous system are identified in which symptoms and/or signs recur. In 10/32, the recurrence of a symptom complex is one of the defining features of the illness, whereas in 22/32 oscillatory signs occur in the setting of an ongoing nervous system disorder. We discuss the possibility that these disorders may be dynamic diseases.
Dynamical Disease: Identification, Temporal Aspects And Treatment Strategies For Human Illness, Jacques Bélair, Leon Glass, Uwe An Der Heiden, John Milton
Dynamical Disease: Identification, Temporal Aspects And Treatment Strategies For Human Illness, Jacques Bélair, Leon Glass, Uwe An Der Heiden, John Milton
WM Keck Science Faculty Papers
Dynamical diseases are characterized by sudden changes in the qualitative dynamics of physiological processes, leading to abnormal dynamics and disease. Thus, there is a natural matching between the mathematical field of nonlinear dynamics and medicine. This paper summarizes advances in the study of dynamical disease with emphasis on a NATO Advanced Research Workshop held in Mont Tremblant, Quebec, Canada in February 1994. We describe the international effort currently underway to identify dynamical diseases and to study these diseases from a perspective of nonlinear dynamics. Linear and nonlinear time series analysis combined with analysis of bifurcations in dynamics are being used …
Strain-Dependent Variation In Carbon Source Regulation Of Nucleus-Encoded Mitochondrial Proteins Of Saccharomyces Cerevisiae., Timothy A. Brown, Bernard L. Trumpower
Strain-Dependent Variation In Carbon Source Regulation Of Nucleus-Encoded Mitochondrial Proteins Of Saccharomyces Cerevisiae., Timothy A. Brown, Bernard L. Trumpower
Dartmouth Scholarship
Nuclear genes encoding mitochondrial proteins are regulated by carbon source with significant heterogeneity among four Saccharomyces cerevisiae strains. This strain-dependent variation is seen both in respiratory capacity of the cells and in the expression of beta-galactosidase reporter fusions to the promoters of CYB2, CYC1, CYC3, MnSOD, and RPO41.
Microwave Oven--Not Only For Cooking, S Pervez
Microwave Oven--Not Only For Cooking, S Pervez
Department of Pathology and Laboratory Medicine
No abstract provided.
Ua66/14/4 Kentucky Partnership Newsletter, Vol. Ii, No. 7, Kentucky Partnership For Farm Family Health & Safety
Ua66/14/4 Kentucky Partnership Newsletter, Vol. Ii, No. 7, Kentucky Partnership For Farm Family Health & Safety
University Organizations
Newsletter created by and about the Kentucky Partnership for Farm Family Health & Safety.
A Novel Translational Regulation Function For The Simian Virus 40 Large-T Antigen Gene., Prithi Rajan, Sathyamagalam Swaminathan, Jiyue Zhu, Charles N. Cole
A Novel Translational Regulation Function For The Simian Virus 40 Large-T Antigen Gene., Prithi Rajan, Sathyamagalam Swaminathan, Jiyue Zhu, Charles N. Cole
Dartmouth Scholarship
Cells use the interferon-induced, double-stranded-RNA-dependent protein kinase PKR as a defense against virus infections. Upon activation, PKR phosphorylates and thereby inactivates the protein synthesis initiation factor eIF-2, resulting in the cessation of protein synthesis. Viruses have evolved various strategies to counteract this cellular defense. In this paper, we show that simian virus 40 (SV40) large-T antigen can antagonize the translational inhibitory effect resulting from the activation of PKR in virus-infected cells. Unlike the situation with other virus-host cell interactions, SV40 large-T antigen does not block the activation of PKR, suggesting that SV40 counteracts the cellular antiviral response mediated by PKR …
Increased Stem Cell Factor Release By Hemangioma-Derived Endothelial Cells, C. J. Meininger, Shannon Brightman, K. A. Kelly, B. R. Zetter
Increased Stem Cell Factor Release By Hemangioma-Derived Endothelial Cells, C. J. Meininger, Shannon Brightman, K. A. Kelly, B. R. Zetter
Biology Faculty Publications
Background: Capillary hemangiomas, the most common tumors in young children, consist of proliferating capillary vessels and endothelial cells. These tumors also contain large numbers of mast cells, compared with the normal surrounding skin or tissue. We have recently shown that stem cell factor (SCF), the gene product of the murine steel locus, can act as a chemoattractant for mast cells. In this study, we investigated whether SCF might be involved in the recruitment and maintenance of mast cells in hemangiomas. Experimental Design: Cultured endothelial cells derived from a murine hemangioma were compared with normal vascular endothelial cells for the ability …
Ua12/12 Wku Wellnews, Vol. 2, No. 2, Wku Wellness Center
Ua12/12 Wku Wellnews, Vol. 2, No. 2, Wku Wellness Center
WKU Archives Records
Newsletter created by the Wellness Center regarding services, health and fitness.
Certain Canine Weakly Β-Hemolytic Intestinal Spirochetes Are Phenotypically And Genotypically Related To Spirochetes Associated With Human And Porcine Intestinal Spirochetosis, Gerald E. Duhamel, Nagaraja Muniappa, Michelle R. Mathiesen, J. L. Johnson, J. Toth, R. O. Elder, A. R. Doster
Certain Canine Weakly Β-Hemolytic Intestinal Spirochetes Are Phenotypically And Genotypically Related To Spirochetes Associated With Human And Porcine Intestinal Spirochetosis, Gerald E. Duhamel, Nagaraja Muniappa, Michelle R. Mathiesen, J. L. Johnson, J. Toth, R. O. Elder, A. R. Doster
School of Veterinary and Biomedical Sciences: Faculty Publications
Four canine weakly β-hemolytic intestinal spirochetes associated with intestinal spirochetosis (IS-associated WBHIS) were compared with IS-associated human and porcine WBHIS and the type species for Serpulina hyodysenteriae and S. innocens by using phenotypic and genotypic parameters. The IS-associated canine, human, and porcine WBHIS belonged to a phyletic group distinct from but related to previously described Serpulina type species.
Sex And Temperament In Modern Society: A Darwinian View Of The “Glass Ceiling” And The “Gender Gap” In Compensation, Kingsley R. Browne
Sex And Temperament In Modern Society: A Darwinian View Of The “Glass Ceiling” And The “Gender Gap” In Compensation, Kingsley R. Browne
Law Faculty Research Publications
No abstract provided.
Pesticide Safety: A Photonovel, M. Susan Jones, The Kentucky Partnership For Farm Family Health & Safety
Pesticide Safety: A Photonovel, M. Susan Jones, The Kentucky Partnership For Farm Family Health & Safety
Nursing Faculty Publications
No abstract provided.
How The Rat Turned White, Kenneth J. Shapiro
How The Rat Turned White, Kenneth J. Shapiro
Experimentation Collection
This is the first in a three-part series on the use of animals in psychological research. In it, I describe how animals got into laboratories in the first place, and their purpose and life there. In the second, I will describe animal model research, the strategy whereby psychologists' develop nonhuman animal models to study human psychopathology. In the concluding piece, I will present a critique of this enterprise, using original data I gathered. The three articles are based on a forthcoming book, Animal Models of Human Psychology: Science, Ethics, and Policy.
Biotransformation Of Bile Acids, Cholesterol And Steroids. Chapter 13 In: The Ecology And Physiology Of Gastrointestinal Microbes. Vol. 1, New York, Chapman And Hall., Stephen Baron, Phillip B. Hylemon
Biotransformation Of Bile Acids, Cholesterol And Steroids. Chapter 13 In: The Ecology And Physiology Of Gastrointestinal Microbes. Vol. 1, New York, Chapman And Hall., Stephen Baron, Phillip B. Hylemon
Biology Faculty Scholarship
No abstract provided.
In Vitro Effects Of Two Silver Electrodes On Select Wound Pathogens, Linda Jean Laatsch-Lybeck, Poh Chye Ong, Luther C. Kloth
In Vitro Effects Of Two Silver Electrodes On Select Wound Pathogens, Linda Jean Laatsch-Lybeck, Poh Chye Ong, Luther C. Kloth
Physical Therapy Faculty Research and Publications
The use of electrical current to promote wound healing is well documented. However, little is understood about the effects of micro-amperage direct current (μADC) on growth of wound pathogens. The purpose of this project was to investigate the antibacterial effects of a silver wire/silver nylon electrode carrying μADC in vitro on several Gram positive and Gram negative bacteria. The current was delivered via silver wire and silver nylon electrodes at an amplitude of 100 μA for a 30-minute duration in an in vitro system. Results demonstrated that only silver wire carrying current inhibited bacterial growth around …