Medicine and Health Sciences Commons^™

Comparison Of A Quantitative Microtiter Method, A Quantitative Automated Method, And The Plate-Count Method For Determining Microbial Complement Resistance, Margie D. Lee, Richard E. Wooley, John Brown, Kathy R. Spears, Lisa K. Nolan, Emmett B. Shotts Jr.

Lisa K. Nolan

A quantitative microtiter method for determining the degree of complement resistance or sensitivity of microorganisms is described. The microtiter method is compared with a quantitative automated system and the standard plate-count technique. Data were accumulated from 30 avian Escherichia coli isolates incubated at 35 C with either chicken plasma or heat-inactivated chicken plasma. Analysis of data generated by the automated system and plate-count techniques resulted in a classification of the microorganisms into three groups: those sensitive to the action of complement; those of intermediate sensitivity to the action of complement; and those resistant to the action of complement. Although the …

Comparison Of Chicken Plasma And Guinea Pig Serum In A Quantitative Microtiter Method Of Determining Microbial Complement Resistance, Richard E. Wooley, John Brown, Kathy R. Spears, Lisa K. Nolan

Lisa K. Nolan

Y. A quantitative microtiter method using chicken plasma is described for determining the degree of complement resistance or sensitivity of avian Escherichia coli isolates. Results obtained with the microtiter method using chicken plasma were compared with results obtained using commercially available standardized guinea pig serum as the source of complement. The test organisms consisted of five isolates of E. coli isolated from chickens. Three isolates were from flocks with colisepticemia; one was from a flock with omphalitis; and one isolate was a non-pathogenic control. Data were accumulated from the five avian E. coli isolates incubated at 35 C with either …

Bronchiolar Metaplasia And Ulex Europaeus Agglutinin I (Uea-I) Affinity In Mycoplasma Hyopneumoniae-Infected Lungs Of Six Pigs, Mark R. Ackermann, M. C. Debey, B. M. Debey

Mark R. Ackermann

Mycoplasma hyopneumoniae adheres to the cilia and apical plasma membrane of cells that line the trachea, bronchi, and bron ch ioles. Colonization result s in ciliostasis and peribronchial and peribronchi olar nod ular aggregates of lymphocytes and plasma cells.">Altho ugh adhesion-receptor interaction s between the organism and respiratory epithelial cells have been examined, little attention has been given to the morphologic and phenotypic changes that may occur in cells lining bron chioles during infection. In this study, we describe morphologic cha nges and differenc es in lectin staining patt ern s in the bron chi oles from lungs of …

Experimental Model Of Atrophic Rhinitis In Gnotobiotic Pigs, Mark R. Ackermann, R. B. Rimler, J. R. Thurston

Mark R. Ackermann

To study the pathogenesis of atrophic rhinitis, gnotobiotic pigs (n = 6) were inoculated intranasally with a sterile sonicate of a toxigenic strain of Bordetella bronchiseptica (0.16 mg of protein per ml) at 5 days of age, and they were then inoculated intranasally with 1 ml (5,250 CFU/ml) of a live, toxigenic strain of Pasteurella multocida at 7 days of age. Pigs were necropsied at 2, 5, 9, 14, 21, and 28 days postinoculation; those pigs necropsied after 5 days had developed turbinate atrophy. Other gnotobiotic pigs received the following inoculation protocols: (i) a sterile sonicate of a nontoxigenic strain …

Interphotoreceptor Retinoid-Binding Protein (Irbp) In Progressive Rod-Cone Degeneration (Prcd)—Biochemical, Immunocytochemical And Immunologic Studies, Gustavo Aguirre, Barbara Wiggert, Geetha Kutty, Kenneth Long, Lila Inouye, Igal Gery, Gerald Chader

Gustavo D. Aguirre, VMD, PhD

Colonization Of The Pharyngeal Tonsil And Respiratory Tract Of The Gnotobiotic Pig By A Toxigenic Strain Of Pasteurella Multocida Type D, Mark R. Ackermann, N. F. Cheville, J. E. Gallagher

Mark R. Ackermann

Seven-day-old gnotobiotic pigs were inoculated intranasally with Pasteurella multocida and euthanatized 2, 5, 9, and 14 days after inoculation. Tissues from the oropharynx and respiratory tract of pigs were cultured quantitatively and analyzed microscopically. Pigs remained afebrile and alert, except one that died of acute fibrinopurulent pneumonia. Pasteurella multocida was isolated in greatest numbers from the pharyngeal tonsils, but only in low numbers from turbinate, trachea, lung, spleen, and liver. Significant histologic changes were limited to the tonsil. Infected pigs developed mild tonsillitis with lymphocytic hyperplasia, and accumulation of cell debris and bacteria in crypts. Capsular antigens of P. multocida, …

The Cone Matrix Sheath In The Normal And Diseased Retina: Cytochemical And Biochemical Studies Of Peanut Agglutinin-Binding Proteins In Cone And Rod-Cone Degeneration, Kenneth Long, Gustavo Aguirre

Gustavo D. Aguirre, VMD, PhD

Glycosaminoglycan And Collagen Metabolism In Arylsulfatase B-Deficient Retinal Pigment Epithelium In Vitro, Gustavo D. Aguirre, Lawrence E. Stramm, W Li, M Haskins

Gustavo D. Aguirre, VMD, PhD

Lectin Histochemistry Of Trachea And Lung Of Healthy Turkeys (Meleagris Gallopavo) And Turkeys With Pneumonia, Mark R. Ackermann, N. F. Cheville, P. G. Detilleux

Mark R. Ackermann

Thirteen lectins were used to characterize lectin-binding specificity of glycoconjugates on sections of formalin-fixed lung and trachea from seven normal turkeys, two turkeys with acute pneumonia, and two turkeys with chronic pneumonia. Neuraminidase was used to digest sialic acid residues. One N-acetylgalactosamine-binding lectin and two N-acetylgalactosamine/galactose-binding lectins stained the apical membrane and cytoplasm of multifocal cells that lined air atria and hyperplastic granular cells. Other lectins in these groups stained ciliated cells of the trachea and bronchi and air capillary epithelial cells. Sialic acid residues were on apical surfaces of ciliated and nonciliated tracheal and bronchial lining cells, air capillary …

Immunity Induced In Rats Vaccinated With Toxoid Prepared From Heat-Labile Toxin Produced By Pasteurella Multocida Serogroup D, J. R. Thurston, R. B. Rimler, Mark R. Ackermann, N. F. Cheville, J. M. Sacks

Mark R. Ackermann

Rats were vaccinated with a toxoid (D-toxoid) prepared from purified heat-labile toxin (D-toxin) produced by Pasteurella multocida serogroup D. Vaccination of rats with D-toxoid prevented death and other effects of D-toxin (hepatic necrosis, development of elevated leukocyte counts, lymphopenia, neutrophilia, and elevated complement titers) that occurred in phosphate buffered saline (PBS)-vaccinated control rats.