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Oncogenic Transformation Of Mammary Epithelial Cells By Transforming Growth Factor Beta Independent Of Mammary Stem Cell Regulation, Karen Dunphy, Jae-Hong Seo, Daniel J. Kim, Amy L. Roberts, Luwei Tao, James Direnzo, Amanda L. Balboni, Giovanna M. Crisi, Mary J. Hagen, Thiruppavai Chandrasekaran, Kelly J. Gauger, Sallie Smith Schneider, D Joseph Jerry
Oncogenic Transformation Of Mammary Epithelial Cells By Transforming Growth Factor Beta Independent Of Mammary Stem Cell Regulation, Karen Dunphy, Jae-Hong Seo, Daniel J. Kim, Amy L. Roberts, Luwei Tao, James Direnzo, Amanda L. Balboni, Giovanna M. Crisi, Mary J. Hagen, Thiruppavai Chandrasekaran, Kelly J. Gauger, Sallie Smith Schneider, D Joseph Jerry
Karen Dunphy
Background: Transforming growth factor beta (TGFβ) is transiently increased in the mammary gland during involution and by radiation. While TGFβ normally has a tumour suppressor role, prolonged exposure to TGFβ can induce an oncogenic epithelial to mesenchymal transition (EMT) program in permissive cells and initiate the generation of cancer stem cells. Our objective is to mimic the transient exposure to TGFβ during involution to determine the persistent effects on premalignant mammary epithelium. Method: CDβGeo cells, a transplantable mouse mammary epithelial cell line, were treated in vitro for 14 days with TGFβ (5 ng/ml). The cells were passaged for an additional …