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Full-Text Articles in Medicine and Health Sciences

Selection Of The Appropriate Reference Genes For Relative Quantitative Reverse Transcription Polymerase Chain Reaction (Qrt-Pcr) In Canine Pulmonary Arteries, Nattawan Tangmahakul, Somporn Techangamsuwan, Sirilak Disatian Surachetpong Dec 2019

Selection Of The Appropriate Reference Genes For Relative Quantitative Reverse Transcription Polymerase Chain Reaction (Qrt-Pcr) In Canine Pulmonary Arteries, Nattawan Tangmahakul, Somporn Techangamsuwan, Sirilak Disatian Surachetpong

The Thai Journal of Veterinary Medicine

A normalisationof targeted genes using the appropriate reference genes is necessary for reliable quantitative reverse transcription polymerase chain reaction (qRT-PCR) experiments. This study aimed to investigate the appropriate reference genes and the numbers of the genes required for qRT-PCR in canine pulmonary arteries. Pulmonary arteries were collected from twenty-three dogs at necropsy. Candidate reference genesincluding ribosomal protein L32 (RPL32), ribosomal protein L13a (RPL13A), ribosomal protein S18 (RPS18), TATA box binding protein (TBP), beta-2-microglobulin (B2M), ribosomal protein S5 (RPS5), ribosomal protein S19 (RPS19) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from pulmonary arteries were examined by qRT-PCR and analysed using four major algorithms, including …


Prevalence Of Canine Parvovirus And Rotavirus With Diarrhea In Western Taiwan, Chiu-Huang Ting, Chia-Ying Lin, Hung-Yi Wu, Hung-Yi Wu, Yueh-Fang Lee, Ching-Dong Chang, Shyh-Shyan Liu Sep 2019

Prevalence Of Canine Parvovirus And Rotavirus With Diarrhea In Western Taiwan, Chiu-Huang Ting, Chia-Ying Lin, Hung-Yi Wu, Hung-Yi Wu, Yueh-Fang Lee, Ching-Dong Chang, Shyh-Shyan Liu

The Thai Journal of Veterinary Medicine

In this study, we investigated the prevalence of Diarrhea pathogens in pet dogs referred to as Canine Parvovirus (CPV) and Canine Rotavirus (CRV)in western Taiwan using conventional polymerase chain reaction (PCR).Fecalsampleswere collected from240 dogs which have the symptomsofdiarrhea examined bythe veterinaryhospitals from March 2015 to March 2017.The PCR sensitivityof total DNAs extracted from 0.1g fecal samples rangedfrom 10ng to100 ng. The prevalence of CPV and CRV infections were23.3% (56/240) and 9.2% (22/240)respectively.The related analysis between prevalence rates and the epidemiological data of pet dogs were correlated with the age, season,area, vaccinationandbreed. The results showed that both the diseases have the …


Two Cases Of Phacoemulsification In The Presence Of A Small Pupil Using An Iris Expander, Ha-Eun Lee, Joon-Young Kim, Da-Eun Lee, Jin-Gu Kang Jan 2019

Two Cases Of Phacoemulsification In The Presence Of A Small Pupil Using An Iris Expander, Ha-Eun Lee, Joon-Young Kim, Da-Eun Lee, Jin-Gu Kang

Turkish Journal of Veterinary & Animal Sciences

A small pupil and poor pupil dilation are well-known risk factors faced by veterinary ophthalmologists during and after cataract surgery. We here describe two cases of phacoemulsification performed using an iris expander (Visitec I-Ring Pupil Expander, Beaver-Visitec International, USA) for treating cataracts in dogs with small pupils. Using this technique, we performed cataract extraction effectively in dogs with insufficiently dilated pupils. This case report provides useful information on the use of an iris expander for phacoemulsification in eyes with a small pupil, which can be applied in veterinary settings.


Proliferation Effects Of Phenylboronic Acid And Boric Acid On Canine Peripheral Blood Mononuclear Cells, Uçkun Sai̇t Uçan, Zafer Sayin Jan 2019

Proliferation Effects Of Phenylboronic Acid And Boric Acid On Canine Peripheral Blood Mononuclear Cells, Uçkun Sai̇t Uçan, Zafer Sayin

Turkish Journal of Veterinary & Animal Sciences

The effects of phenylboronic acid (PBA) and boric acid (BA) on the proliferation of canine peripheral blood mononuclear cells (PBMCs) were investigated. Treatment of PBMCs from mixed breed dogs with PBA or BA (0.05 µg/mL) alone elicited blastogenic responses of 0.478 ± 0.438 and 0.499 ± 0.366, respectively. We observed an additive effect on proliferation when purebred Kangal PBMCs were cultured in a combination of BA (0.001 µg/mL) and the mitogenic lectin concanavalin A (1 µg/mL). However, the effects were not statistically significant. We detected varied IL-2 and IL-4 secretion in response to stimulation with boron-containing compounds. Our findings suggest …