Medicine and Health Sciences Commons^™

Synergistic Effects Of Methylglyoxal And Hyperglycemia On Ros Generation And The Viability Of Cultured H9c2 Myoblast Cells, Robinderpal Sandhu, Dylan Lefkovitz, Al Ibe, Alisa Kim, Peter Wieczorek, Dean Delgado, Robert J. Barsotti, Lindon H. Young, Qian Chen

Research Day

Heart damage in diabetics may be closely related to the possible synergistic cellular damage from hyperglycemia and increased methylglyoxal levels. This study investigated the effects of glucose and/or methylglyoxal and/or metformin on H9c2 reactive oxygen species (ROS) generation measured by a dichlorofluorescein diacetate (DCFDA) assay and cell viability measured by a cell counting kit-8 assay after various treatments for 24 hours.

Glucose treatment (5 mM-40 mM) displayed similar cell viability (n=4) and ROS generation (n=7) when compared to control cells. By contrast, methylglyoxal (5 µM-1400 µM) decreased cell viability at higher concentration (1000 µM (51 ± 8%); 1200 µM (41 …

Identifying Motivators And Barriers For Wellness Programs To Inform Recruitment And Retention Of Diabetes Prevention Programs (Dpps), Beenish Kamran, Kate Beatty, Leighanne Hurst, Deborah L. Slawson

Appalachian Student Research Forum

Obesity and type 2 diabetes (T2DM) has increased greatly in incidence in the United States over the past decade. Diagnosis of T2DM is typically preceded by insulin resistance, which is typically indicated by increases in fasting blood sugar and called pre-diabetes. The Diabetes Prevention Program (DPP) is a group-based program focused on improving diet and maintaining consistent exercise towards a lower body weight. It has been found that minimum weight loss and 150 minutes of moderate exercise is more effective at preventing T2DM than medication-based treatment. Therefore, the DPP has potential in preventing T2DM however currently faces obstacles concerning recruitment …