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Full-Text Articles in Medicine and Health Sciences
Nmr Studies Of Acyl Chain-Protein Interactions, Mark Christian Oswood
Nmr Studies Of Acyl Chain-Protein Interactions, Mark Christian Oswood
Yale Medicine Thesis Digital Library
An approach employing heteronuclear NMR is presented for monitoring the interactions between long-chain fatty acids and proteins which are involved in their synthesis and intracellular transport. Two naturally occurring fatty acids, hexadecanoic (palmitic) and cis-9-10-methylene-hexadecanoic (MHA) acids were labeled using a biosynthetic scheme with 13C acetate as a source. The biosynthetic incorporation of 13C from a two carbon source resulted in sequential pairs of 13C atoms in the product. When combined with double quantum spectroscopy, a highly efficient suppression of natural abundance background resonances results. Proton-detected two and three-dimensional experiments which correlate double quantum carbon chemical shifts with proton shifts …
Genetic And Biochemical Characterization Of Drosophila Singed, A Homolog Of The Actin Bundling Protein Fascin, Kelly Cant
Yale Medicine Thesis Digital Library
Drosophila singed mutants were named for their gnarled bristle phenotype, however, severe alleles are also female sterile due to a defect in nurse cell cytoplasm transport. Recently, singed protein was shown to have 35% peptide identity with the actin bundling protein fascin. I present evidence that singed is a homolog of fascin. In vivo, singed is required for actin filament bundle formation in the cytoplasm of nurse cells during oogenesis and in the cellular extension that forms a bristle. In vitro, purified bacterially-expressed singed bundles actin filaments with the same 12 nm periodicity and stoichiometry reported for sea urchin fascin.While …
The Cloning And Characterization Of Toad-64: A Gene Expressed Transiently During Neuronal Differentiation In The Mammalian Nervous System, Jane Elizabeth Minturn
The Cloning And Characterization Of Toad-64: A Gene Expressed Transiently During Neuronal Differentiation In The Mammalian Nervous System, Jane Elizabeth Minturn
Yale Medicine Thesis Digital Library
During the development of the mammalian central nervous system, a pool of morphologically homogeneous, mitotically active progenitor cells in the neural tube gives rise to the enormously diverse population of postmitotic cells that assume the properties of neurons or glia. Postmitotic neurons elaborate processes that are involved in the migration of neurons to their adult positions as well as the elaboration of axonal and dendritic arbors that will ultimately form specific synaptic contacts. The generation of postmitotic neurons from precursor cells is a central differentiative event in development, but molecular markers of this event are few.Using 2D gel electrophoresis, proteins …
Perceptions Of Vulnerability And Fears Of Aids: A Survey Of Elementary School Children, John S. Maypole
Perceptions Of Vulnerability And Fears Of Aids: A Survey Of Elementary School Children, John S. Maypole
Yale Medicine Thesis Digital Library
No abstract provided.
Photochemotherapy Of Vascular Cells With 8-Methoxypsoralen And Visible Light: A Novel Approach To The Prevention Of Post-Angioplasty Restenosis, David Marshall Lee
Photochemotherapy Of Vascular Cells With 8-Methoxypsoralen And Visible Light: A Novel Approach To The Prevention Of Post-Angioplasty Restenosis, David Marshall Lee
Yale Medicine Thesis Digital Library
No abstract provided.
Characterization Of Two Endogenous Trophic Factors Involved In Regeneration Of The Goldfish Optic Nerve, Jason Matthew Schwalb
Characterization Of Two Endogenous Trophic Factors Involved In Regeneration Of The Goldfish Optic Nerve, Jason Matthew Schwalb
Yale Medicine Thesis Digital Library
Unlike mammals, lower vertebrates are able to regenerate injured pathways of the central nervous system throughout life. The factors responsible for initiating this process are unknown. We have shown that in dissociated cell culture, goldfish retinal ganglion cells regenerate their axons in response to two factors derived from goldfish optic nerve glia. Axogenesis factor 1 (AF-1) is a small, hydrophilic peptide (700-900 daltons) that is inactivated by treatment with proteinase K, but heat-stable. A second factor, AF-2, is a basic polypeptide of c. 12kDa. Dissociated retinal cells remain viable in serum-free defined media for at least a week, but show …