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Full-Text Articles in Medicine and Health Sciences
The Mechanism Of T Cell Dysfunction Induced By Diethylstilbestrol, Nicole Chantae Brown
The Mechanism Of T Cell Dysfunction Induced By Diethylstilbestrol, Nicole Chantae Brown
Theses and Dissertations
Estrogens have the ability to alter the immune system. Diethylstilbestrol (DES), asynthetic estrogen, is known to have estrogenic activity and induce thymic alterations.We investigated the mechanism by which DES is able to alter T cells and thus theimmune system. First, we studied the effect of DES on mature T cells by using the T cellleukemia cell line, Jurkat. We found that DES treatment reduced cell viability andincreased apoptosis. Additionally, apoptosis was found to involve both death receptorand mitochondria1 pathways. Furthermore, estrogen receptor beta was found to beexpressed in these cells and increased following DES treatment. Secondly, we studiedthe effect of …
Ischemic Preconditioning Protects Adult Rat Cardiomyocytes Against Necrosis But Not Apoptosis, Via Activation Of Pkg, Marc J. Caligtan
Ischemic Preconditioning Protects Adult Rat Cardiomyocytes Against Necrosis But Not Apoptosis, Via Activation Of Pkg, Marc J. Caligtan
Theses and Dissertations
The role of cyclic guanosine monophosphate (cGMP) dependent protein kinase (PKG) in necrotic and apoptotic pathways of many cell types is well established; however its role in the ischemic preconditioning (IPC) of cardiomyocytes is not clearly defined. In the current study, we assessed the hypothesis that PKG protects against cell death following ischemidreperfusion injury in myocytes subjected to IPC. Freshly isolated adult rat ventricular myocytes were subjected to IPC by incubating in ischemic buffer for 30 minutes (min) followed by incubation in normal medium for 30 min. Prolonged simulated ischemia (SI) was created by incubating myocytes in the ischemic buffer …