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Evaluation Of A Multi-Marker Immunomagnetic Enrichment Assay For The Quantification Of Circulating Melanoma Cells, James Freeman, Elin Gray, Michael Milward, Robert Pearce, Melanie Ziman
Evaluation Of A Multi-Marker Immunomagnetic Enrichment Assay For The Quantification Of Circulating Melanoma Cells, James Freeman, Elin Gray, Michael Milward, Robert Pearce, Melanie Ziman
Research outputs 2012
Background: Circulating melanoma cells (CMCs) are thought to be valuable in improving measures of prognosis in melanoma patients and may be a useful marker of residual disease to identify non-metastatic patients requiring adjuvant therapy. We investigated whether immunomagnetic enrichment targeting multiple markers allows more efficient enrichment of CMCs from patient peripheral blood than targeting a single marker. Furthermore, we aimed to determine whether the number of CMCs in patient blood was associated with disease stage.Methods: We captured CMCs by targeting the melanoma associated markers MCSP and MCAM as well as the melanoma stem cell markers ABCB5 and CD271, both individually …