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Medicine and Health Sciences Commons

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Jonathan Tan

2014

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Full-Text Articles in Medicine and Health Sciences

Spleen Stroma Maintains Progenitors And Supports Long-Term Hematopoiesis, Helen O'Neill, Kristin Griffiths, Pravin Periasamu, Rebecca Hinton, Sawang Petvises, Ying-Ying Hey, Jonathan Tan Dec 2013

Spleen Stroma Maintains Progenitors And Supports Long-Term Hematopoiesis, Helen O'Neill, Kristin Griffiths, Pravin Periasamu, Rebecca Hinton, Sawang Petvises, Ying-Ying Hey, Jonathan Tan

Jonathan Tan

Hematopoietic stem/progenitor cells (HSPC) differentiate in the context of stromal niches producing cells of multiple lineages. Limited success has been achieved in the past with induction of hematopoiesis in vitro. Previously, spleen long-term stromal cultures (LTC) were shown to continuously support restricted hematopoiesis for production of novel dendritic-like cells (LTC-DC). An in vivo equivalent dendritic cell type was then described which is specific for spleen. The in vivo counterpart cell was termed 'L-DC' and represents a dendritic-like CD11cloCD11bhiCD8a-MHC-II- cell which differs phenotypically and functionally from monocytes/macrophages and conventional and plasmacytoid DC. Splenic stroma is now shown to maintain HSPC and …


Characterisation Of The Effect Of Lps On Dc Subset Discrimination In Spleen., Kristin Griffiths, Jonathan Tan, Helen O'Neill Dec 2013

Characterisation Of The Effect Of Lps On Dc Subset Discrimination In Spleen., Kristin Griffiths, Jonathan Tan, Helen O'Neill

Jonathan Tan

The Gram-negative bacterial endotoxin lipopolysaccharide (LPS) is a potent inflammatory mediator and a leading cause of bacterial sepsis. While LPS is known to activate antigen-presenting cells, here we find that LPS down-regulates expression of CD11c and CD11b on splenic dendritic cell subsets, thus confounding the ability to identify these subsets following treatment. This has implications with regard to tracking the response to LPS in terms of the cell subsets involved, and should be considered whenever such studies are undertaken.