Medicine and Health Sciences Commons^™

Myelopoiesis In Spleen Producing Distinct Dendritic-Like Cells, Jonathan Tan, Helen O'Neill

Jonathan Tan

Dendritic cells (DC) represent a heterogeneous class of antigen presenting cells (APC). Previously we reported a distinct myeloid dendritic-like cell present in spleen, as an in vivo counterpart to cells produced in murine spleen long-term cultures (LTC-DC). These cells, named 'L-DC', were found to be functionally and phenotypically distinct from conventional (c)DC, plasmacytoid (p)DC and monocytes. These results suggested that spleen may represent a niche for development of L-DC from endogenous progenitors. Adult murine spleen has now been investigated for the presence of L-DC progenitors. Lineage-negative (Lin)-ckitlo and Lin-ckithi progenitor subsets were identified as candidate populations, and tested for ability …