Open Access. Powered by Scholars. Published by Universities.®
Electronic Thesis and Dissertation Repository
Analytical, Diagnostic and Therapeutic Techniques and Equipment
Articles 1 - 2 of 2
Full-Text Articles in Medicine and Health Sciences
Hepcidin-Mediated Iron Regulation In P19 Cells Is Detectable By Mri, Kobra Alizadeh Pourbouyeh
Hepcidin-Mediated Iron Regulation In P19 Cells Is Detectable By Mri, Kobra Alizadeh Pourbouyeh
Electronic Thesis and Dissertation Repository
Magnetic resonance imaging (MRI) can be used to track cellular activities in the body using iron-based contrast agents. However, intrinsic cellular iron handling mechanisms may also influence the detection of magnetic resonance (MR) contrast. For instance, inflammation involves downregulation of iron export in macrophages by the hormone hepcidin, due to degradation of the iron export protein, ferroportin (Fpn). We examined the effect of hepcidin on iron regulation and MR transverse relaxation rates in multi-potent P19 cells, which display high iron export activity, similar to macrophages. In response to varying conditions of iron supplementation, our results showed similar Fpn expression in …
Evaluating Evolving Leukocyte Populations In Peripheral Blood Circulation Post-Concussion In A Human Longitudinal Analysis Of Female Athletes, Kevin A. Blackney
Evaluating Evolving Leukocyte Populations In Peripheral Blood Circulation Post-Concussion In A Human Longitudinal Analysis Of Female Athletes, Kevin A. Blackney
Electronic Thesis and Dissertation Repository
Concussions are generating increasing concern due to potential long-term neurological consequences. Currently there is no universally recognized diagnostic approach for concussion. I hypothesize that a signature temporal response of biomarkers of inflammation in systemic circulation will provide an objective diagnosis of concussion and could also be used to track patient recovery. The Western University women’s rugby team underwent blood draws at pre-season and post-season as a baseline evaluation, and players determined to have sustained a concussion underwent repeat blood analysis post-concussion. Blood samples were analyzed by flow cytometry to profile immune cell populations alongside accepted concussion assessments, and complete blood …