Medicine and Health Sciences Commons^™

Pulmonary And Systemic Pathophysiological Impact Of Silver Nanoparticles: Effects Of Coating, Time, And Dose, Zannatul Ferdous

Dissertations

Nanotechnology is rapidly developing, and engineered materials (ENMs) are utilized in a wide array of end-user products owing to their unique physicochemical properties. Of the various forms of ENMs, silver nanoparticles (AgNPs) have gained much attention for potential uses in commercial applications. Due to their unique features, particularly antimicrobial properties, there had been a surge in the production of AgNPs-based products, hence increasing the potential of both occupational and consumer exposure. Among the various routes of human exposure, pulmonary exposure to AgNPs has received increased interest due to their potential to cause pulmonary injury, cross the alveolar-capillary barrier, and distribute …

Exogenous Alpha-Synuclein Induces Cell Death Related Proteins In C6 Oligodendrocyte-Like Cells Corresponding To Protein Expression Observed In Multiple System Atrophy, Derrick Samuel Hilton

Dissertations

Multiple System Atrophy (MSA) consists of three disorders; Autonomic Dysfunction, Cerebellar Ataxia, and Parkinsonism. In MSA, the protein Alpha-Synuclein (SNCA) appears in the central nervous system as misfolded protein aggregates primarily in oligodendrocytes. This dissertation reports the results from studies examining the effect of exogenous SNCA has on a cell model: C6 oligodendrocyte-like cells. Treated cells were evaluated using western blot and DNA microarray. In addition the expression of proteins was evaluated using immunocytochemistry in MSA patient tissue.

C6 cells were shown to take up SNCA when added to the media. SNCA also underwent a truncation when taken up by …