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Full-Text Articles in Medicine and Health Sciences
Three V-Snares And Two T-Snares, Present In A Pentameric Cis-Snare Complex On Isolated Vacuoles, Are Essential For Homotypic Fusion, Christian Ungermann, Gabriele F. Von Mollard, Ole N. Jensen, Nathan Margolis, Tom H. Stevens, William Wickner
Three V-Snares And Two T-Snares, Present In A Pentameric Cis-Snare Complex On Isolated Vacuoles, Are Essential For Homotypic Fusion, Christian Ungermann, Gabriele F. Von Mollard, Ole N. Jensen, Nathan Margolis, Tom H. Stevens, William Wickner
Dartmouth Scholarship
Vacuole SNAREs, including the t-SNAREs Vam3p and Vam7p and the v-SNARE Nyv1p, are found in a multisubunit "cis" complex on isolated organelles. We now identify the v-SNAREs Vti1p and Ykt6p by mass spectrometry as additional components of the immunoisolated vacuolar SNARE complex. Immunodepletion of detergent extracts with anti-Vti1p removes all the Ykt6p that is in a complex with Vam3p, immunodepletion with anti-Ykt6p removes all the Vti1p that is complexed with Vam3p, and immunodepletion with anti-Nyv1p removes all the Ykt6p in complex with other SNAREs, demonstrating that they are all together in the same cis multi-SNARE complex. After priming, which disassembles …
Pregnant Rat Uterus Expresses High Levels Of The Type 3 Iodothyronine Deiodinase, Valerie Anne Galton, Elena Martinez, Arturo Hernandez, Emily A. St. Germain, Joanne M. Bates, Donald L. St. Germain
Pregnant Rat Uterus Expresses High Levels Of The Type 3 Iodothyronine Deiodinase, Valerie Anne Galton, Elena Martinez, Arturo Hernandez, Emily A. St. Germain, Joanne M. Bates, Donald L. St. Germain
Dartmouth Scholarship
Although thyroid hormones are critically important for the coordination of morphogenic processes in the fetus and neonate, premature exposure of the embryo to levels of the hormones present in the adult is detrimental and can result in growth retardation, malformations, and even death. We report here that the pregnant rat uterus expresses extremely high levels of the type 3 iodothyronine deiodinase (D3), which inactivates thyroxine and 3,3′,5-triiodothyronine by 5-deiodination. Both D3 mRNA and activity were present at the implantation site as early as gestational day 9 (E9), when expression was localized using in situ hybridization to uterine mesometrial and antimesometrial …