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Full-Text Articles in Medicine and Health Sciences
The Nascent-Polypeptide-Associated Complex: Having A "Nac" For Fidelity In Translocation., William Wickner
The Nascent-Polypeptide-Associated Complex: Having A "Nac" For Fidelity In Translocation., William Wickner
Dartmouth Scholarship
No abstract provided.
Asbestos Induces Nuclear Factor Kappa B (Nf-Kappa B) Dna-Binding Activity And Nf-Kappa B-Dependent Gene Expression In Tracheal Epithelial Cells., Yvonne M. Janssen, Aaron Barchowsky, Melinda Treadwell, Kevin E. Driscoll, B T. Mossman
Asbestos Induces Nuclear Factor Kappa B (Nf-Kappa B) Dna-Binding Activity And Nf-Kappa B-Dependent Gene Expression In Tracheal Epithelial Cells., Yvonne M. Janssen, Aaron Barchowsky, Melinda Treadwell, Kevin E. Driscoll, B T. Mossman
Dartmouth Scholarship
Nuclear factor kappa B (NF-kappa B) is a transcription factor regulating expression of genes intrinsic to inflammation and cell proliferation--features of asbestos-associated diseases. In studies here, crocidolite asbestos caused protracted and dose-responsive increases in proteins binding to nuclear NF-kappa B-binding DNA elements in hamster tracheal epithelial (HTE) cells. This binding was modulated by cellular glutathione levels. Antibodies recognizing p65 and p50 protein members of the NF-kappa B family revealed these proteins in two of the DNA complexes. Transient transfection assays with a construct containing six NF-kappa B-binding DNA consensus sites linked to a luciferase reporter gene indicated that asbestos induced …
Protein-Peptide Interactions Analyzed With The Yeast Two-Hybrid System, Meijia Yang, Zining Wu, Stanley Fields
Protein-Peptide Interactions Analyzed With The Yeast Two-Hybrid System, Meijia Yang, Zining Wu, Stanley Fields
Dartmouth Scholarship
The yeast two-hybrid system was used to screen a library of random peptides fused to a transcriptional activation domain in order to identify peptides capable of binding to the retinoblastoma protein (Rb). Seven peptides were identified, allof which contain the Leu-X-Cys-X-Glu motif found in Rb-binding proteins, although their activity in the yeast assay variedover a 40-fold range. Mutagenesis of the DNA encoding two of these peptides followed by screening in the two-hybrid systemallowed the delineation of residues apart from the invariant Leu, Cys and Glu that affect binding to Rb. Binding affinities of a peptide and one of its variants …
The Gtp-Bound Form Of The Yeast Ran/Tc4 Homologue Blocks Nuclear Protein Import And Appearance Of Poly(A)+ Rna In The Cytoplasm., Gabriel Schlenstedt, Claudio Saavedra, Jonathan D. Loeb, Charles N. Cole, Pamela A. Silver
The Gtp-Bound Form Of The Yeast Ran/Tc4 Homologue Blocks Nuclear Protein Import And Appearance Of Poly(A)+ Rna In The Cytoplasm., Gabriel Schlenstedt, Claudio Saavedra, Jonathan D. Loeb, Charles N. Cole, Pamela A. Silver
Dartmouth Scholarship
Ran/TC4, a Ras-like GTP-binding protein, and its nucleotide exchanger, RCC1, have been implicated in control of protein movement into the nucleus and cytoplasmic accumulation of mRNA. Saccharomyces cerevisiae contains two homologues of the mammalian Ran/TC4, encoded by the GSP1 and GSP2 genes. We have constructed yeast strains that overproduce either wild-type Gsp1 or a form of Gsp1 with glycine-21 converted to valine (Gsp1-G21V), which we show stabilizes the GTP-bound form. Cells producing Gsp1-G21V have defects in localization of nuclear proteins; nuclear proteins accumulate in the cytoplasm following galactose induction of Gsp1-G21V. Similarly, cells producing Gsp1-G21V retain poly(A)+ RNA in their …