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Full-Text Articles in Medicine and Health Sciences
Prediction Of Future Caries In Toddlers Via Salivary Microbiome: A 1-Year Longitudinal Study, Ratcha Raksakmanut
Prediction Of Future Caries In Toddlers Via Salivary Microbiome: A 1-Year Longitudinal Study, Ratcha Raksakmanut
Chulalongkorn University Theses and Dissertations (Chula ETD)
Dental caries in children is the most common chronic oral disease that could disturb their quality of life including their development. Early prevention is a key approach to reducing the prevalence of early childhood caries. However, a reliable caries predictor, as an essential tool for targeted prevention that is important to this approach, is still lacking for infants before caries onset. Therefore, we aimed to develop the caries risk prediction model based on the salivary microbiome of caries-free 1-year-old children to predict caries onset at 1-year follow-up. Using a nested case-control design within a prospective cohort study, 30 saliva samples, …
The Effects Of Decellularized Extracellular Matrix Derived From Jagged1-Treated Human Dental Pulp Stem Cells On Biological Responses Of Stem Cells Isolated From Apical Papilla, Suphalak Phothichailert
The Effects Of Decellularized Extracellular Matrix Derived From Jagged1-Treated Human Dental Pulp Stem Cells On Biological Responses Of Stem Cells Isolated From Apical Papilla, Suphalak Phothichailert
Chulalongkorn University Theses and Dissertations (Chula ETD)
Objective: Indirect Jagged1 immobilization efficiently activates canonical Notch signaling in hDPSCs. This study aimed to investigate the characteristic of the Jagged1-treated hDPSCs-derived dECM and its biological activity on odonto/osteogenic differentiation SCAPs. Methods: Bioinformatic database of Jagged1-treated hDPSCs was analyzed by NetworkAnalyst. hDPSCs seeded on Jagged1 immobilized surface were maintained with N or OM followed by decellularization procedure, dECM-N or dECM-OM, respectively. SCAPs were reseeded on each dECM with either normal medium or OM. Cell viability was determined by MTT assay. Characteristics of dECMs and SCAPs were evaluated by SEM, EDX, immunofluorescent staining and alcian blue staining. A Multipotential differentiation assay. …
Anti-Inflammatory And Regeneration Properties Of Iloprost On Inflamed Human Dental Pulp Cells Model: An In Vitro Study, Rahman Wahyudi
Anti-Inflammatory And Regeneration Properties Of Iloprost On Inflamed Human Dental Pulp Cells Model: An In Vitro Study, Rahman Wahyudi
Chulalongkorn University Theses and Dissertations (Chula ETD)
In the clinical situation, pulp exposure can happen during caries removal procedures. Inflammation can occur on the pulp of the exposed area because of infiltration of bacteria from caries or as a result of pulp reaction to caries removal drilling. When the pulp was exposed during the caries removal procedure, traditional methods to preserve the pulp such as pulp capping and pulpotomy were performed. The ideal treatment outcome of the pulp exposure is to regain the primary structure of tubular dentin as well as maintain the vitality and healthiness of the dental pulp Thus, good dental materials or drugs used …
Role Of Extracellular Adenosine Triphosphate (Eatp) On Immunomodulatory Function Of Human Periodontal Ligament Cells, Maythwe Kyawsoewin
Role Of Extracellular Adenosine Triphosphate (Eatp) On Immunomodulatory Function Of Human Periodontal Ligament Cells, Maythwe Kyawsoewin
Chulalongkorn University Theses and Dissertations (Chula ETD)
Background: Adenosine triphosphate, a nucleotide that acts as an important extracellular messenger, is released into the extracellular environment during various physiologic and pathological conditions. Human periodontal ligament cells (hPDLCs) can release adenosine triphosphate into the extracellular environment in response to mechanical stress. Extracellular adenosine triphosphate (eATP) plays role in both inflammation and osteogenic differentiation processes. eATP also participates in immunosuppressive action on immune cells. However, the role of eATP on the immunomodulatory function of hPDLCs is still unclear. This study aims to investigate the effects of eATP on the immunomodulatory function of hPDLCs and the participation of specific purinergic P2 …
Effect Of Shear Force On Immunosuppressive Property Of Human Periodontal Ligament Cells, Ravipha Suwittayarak
Effect Of Shear Force On Immunosuppressive Property Of Human Periodontal Ligament Cells, Ravipha Suwittayarak
Chulalongkorn University Theses and Dissertations (Chula ETD)
The immunomodulatory property is to regulate immune cell function. IDO and TGF-β1 inhibited T cell proliferation and induced regulatory T (Treg) cells differentiation. However, this property must be activated by inflammation. Recently, mechanical forces have also activated this property. Hence, this study investigated effect of shear stress on immunosuppressive property of hPDL cells. Cells were subjected to shear stress then expression of IDO, TGF-β1, IFN-gamma and COX2 mRNA and protein was examined by qRT-PCR, ELISA, and IDO activity. Conditioned media derived shear stress were treated with CD4+ T cells. The proliferative T cell effect was determined using resazurin. The FOXP3, …
Comparative Gene Expression Analysis Of Human Dental Pulp Cells From Maxillary And Mandibular Teeth, Thira Faruangsaeng
Comparative Gene Expression Analysis Of Human Dental Pulp Cells From Maxillary And Mandibular Teeth, Thira Faruangsaeng
Chulalongkorn University Theses and Dissertations (Chula ETD)
Objective : Given the anatomical and locational differences between the maxillary and mandibular teeth, it is possible that there are differences in gene expression patterns of dental pulp cells within those teeth. This study evaluates and compares the gene expression profiles of the maxillary and mandibular human teeth with the aim of finding differences in gene expression patterns of dental pulp cells by RNA-Sequencing technique. Methods : The upper and lower premolar and molar teeth obtained from the same participant were employed. Two pairs of opposing premolar teeth and two pairs of opposing molar teeth were isolated, cultured, and subjected …
Histone Deacetylase Inhibitor Induces The Odontogenic Differentiation In Human Dental Pulp Cells: Comparison Of Trichostatin A (Tsa) And Suberoylanilide Hydroxamic Acid (Saha), Indrani Sulistyowati
Histone Deacetylase Inhibitor Induces The Odontogenic Differentiation In Human Dental Pulp Cells: Comparison Of Trichostatin A (Tsa) And Suberoylanilide Hydroxamic Acid (Saha), Indrani Sulistyowati
Chulalongkorn University Theses and Dissertations (Chula ETD)
Human dental pulp cells (hDPCs) have shown their plasticity to differentiate into odontoblast-like cell lineages under the treatment of two-members of hydroxamates HDAC inhibitors (HDACis), Trichostatin A (TSA) and Suberoylanilide hydroxamic acid (SAHA). However, a comparison of the potency for stimulating odontoblast-like differentiation and mineralization process among these two HDACis has not been reported. Therefore, we aimed to confirm and compare the stimulatory effect of TSA and SAHA in inducing odontoblast-like differentiation and promoting mineralized-nodule formation. The primary cultured hDPCs was used for MTT assay, ALP activity assay, and alizarin red staining in the presence and absence of TSA and …
Biological Effects Of Epigallocatechin Gallate During The Protection Of The Submandibular Gland After Injury, Erni Sulistiyani
Biological Effects Of Epigallocatechin Gallate During The Protection Of The Submandibular Gland After Injury, Erni Sulistiyani
Chulalongkorn University Theses and Dissertations (Chula ETD)
Background: When radiotherapy is delivered to head and neck cancer (HNC) patients, the salivary gland (SG) secretory epithelia can be irreversibly injured in up to 60% of the individuals, leading to dry mouth or xerostomia. Radiotherapy’s effectiveness in suppressing HNC growth is correlated with an increase in free radicals that produce DNA damage to the tumor and neighboring organs like the SG. Epigallocatechin gallate (EGCG) is one of the most abundant polyphenols present in green tea leaves and a well-known antioxidant. In previous in vitro study using genetically modified immortal SG cell lines, EGCG protected SG cells from γ-radiation. However, …
Bcl-Xl Expression In Osteoclasts Of Patients With Medication-Related Osteonecrosis Of The Jaw: A Comparison With Osteoradionecrosis And Osteomyelitis, Vy Tran Ngoc Thuy
Bcl-Xl Expression In Osteoclasts Of Patients With Medication-Related Osteonecrosis Of The Jaw: A Comparison With Osteoradionecrosis And Osteomyelitis, Vy Tran Ngoc Thuy
Chulalongkorn University Theses and Dissertations (Chula ETD)
Objective: The pathological mechanism of medication-related osteonecrosis of the jaw (MRONJ) is still unknown now. Osteoclasts are cells directly influenced by MRONJ, which might be the key mediator of pathological mechanism. This study aimed to evaluate the histological features of MRONJ, investigate the morphology and quantity of osteoclasts in MRONJ as well as expression of Bcl-xl, and compare it with ORN, OM, and normal jaw bone. Methods: In this study, 57 subjects, including patients with MRONJ, osteoradionecrosis of the jaw (ORN), osteomyelitis of the jaw (OM), and normal jaw bone were studied. Hematoxylin and eosin-stained slides of these diagnosed cases …
Treatment Effects Of Amitriptyline On Pain Symptoms, Quality Of Life And Heart Rate Variability In Burning Mouth Syndrome Patients, Chanida Chaiworn
Treatment Effects Of Amitriptyline On Pain Symptoms, Quality Of Life And Heart Rate Variability In Burning Mouth Syndrome Patients, Chanida Chaiworn
Chulalongkorn University Theses and Dissertations (Chula ETD)
The objectives of this research project were 1) To evaluate the effectiveness of amitriptyline therapy on improving pain and the oral health-related quality of life (OHRQoL) in BMS patients when compared to palliative topical therapies (sodium bicarbonate mouthwash). 2) To determine the association between therapy of amitriptyline and heart rate variability (HRV) parameters. 3) To assess the association between pain outcomes and fluctuations in HRV parameters. This project was composed of a retrospective and a prospective study, and subjects were primary BMS patients recruited at the Oral Medicine Clinic, Faculty of Dentistry, In the retrospective study, 20 female participants were …
Pd-1 Expression On Immune Cells In Sepsis Mouse Model And The Susceptibility To Secondary Fungal Infection, Chau Tran Bao Vu
Pd-1 Expression On Immune Cells In Sepsis Mouse Model And The Susceptibility To Secondary Fungal Infection, Chau Tran Bao Vu
Chulalongkorn University Theses and Dissertations (Chula ETD)
Objectives: This study aimed to i) determine the kinetic changes of immune cell phenotypes, including an exhaustion marker PD-1, in spleens of murine sepsis ii) investigate the susceptibility against secondary fungal infection after sepsis, and iii) investigate the efficacy of an anti-PD-1 treatment in post-sepsis fungal infection. Methods: Cecal ligation and puncture (CLP) was used as a sepsis model and splenocytes post-CLP were assessed by flow cytometry. In addition, secondary post sepsis systemic fungal infections by Candida albicans or Aspergillus fumigatus administration at 5-day post-CLP were performed. Moreover, secondary aspergillosis was treated with Amphotericin B with or without anti-PD-1 to …
Tlr3 Signaling Modulates Immunomodulatory Property Of Human Periodontal Ligament Stem Cells., Daneeya Chaikiawkeaw
Tlr3 Signaling Modulates Immunomodulatory Property Of Human Periodontal Ligament Stem Cells., Daneeya Chaikiawkeaw
Chulalongkorn University Theses and Dissertations (Chula ETD)
Toll like receptor 3 (TLR3) is a member of TLR family functions to recognizes double-stranded RNA (dsRNA) produced by positive-strand RNA viruses and DNA virusesand generate signaling that stimulate body immune system against virus infection. However, recent evidence suggests that TLR3 signaling may also play a role in initiating the immunosuppressive property of the cells. Study on skin regeneration has shown the anti-inflammatory responses of keratinocytes required for wound healing andthe increasing number of hair follicle formation after TLR3 signaling was activated by fragment of RNA released from damaged cells. Moreover, activation of TLR3 signaling have shown to elicit a …
Effect Of Cyclic Tensile Force On The Expression Of Bone Morphogenetic Protein 9 And Interleukin 6 In Human Periodontal Ligament Cells, Yanee Tantilertanant
Effect Of Cyclic Tensile Force On The Expression Of Bone Morphogenetic Protein 9 And Interleukin 6 In Human Periodontal Ligament Cells, Yanee Tantilertanant
Chulalongkorn University Theses and Dissertations (Chula ETD)
Periodontium is a specialized tissue laying between tooth and surrounding bone. It functions as force cushion. In particular stimulation including mechanical force, periodontal ligament (PDL) cells which are mechanosensitive cells have the ability to secrete specific cytokines and proteins. PDL cells also have the potential to differentiate into osteoblast-like cells or fibroblast-like cells. PDL cells perceive combination of force in oral cavity in which major component is cyclic tensile force (CTF). CTF play an essential role in modulating PDL cells to maintain both hard and soft tissue integrity. Here, we demonstrated that CTF was able to stimulate the expression of …
The Application Of Prostacyclin In Promoting Angiogenesis Of Dental Pulp Tissue In A Three Dimensional Organ Culture System, Sonntana Seang
The Application Of Prostacyclin In Promoting Angiogenesis Of Dental Pulp Tissue In A Three Dimensional Organ Culture System, Sonntana Seang
Chulalongkorn University Theses and Dissertations (Chula ETD)
Background: Dental pulp vitality can be threatened by carious or iatrogenic exposure that can lead to pulp tissue loss. The formation of a vascular network is crucial to provide a suitable environment for tissue regeneration. Thus, promoting angiogenesis is essential for successful pulpal repair in regenerative endodontics therapy. Iloprost, a prostacyclin analog, promotes vascularization in several organs such as, heart, lung, and corneal. Previously, it has been demonstrated that, iloprost induced pulpal blood flow and enhanced dentin formation in a rat mechanical pulp exposure model. However, the angiogenic effect of iloprost on the human dental pulp vasculature remains unknown. Objective: …
Induction Of S100a7 Expression From Mechanical Stress-Induced Human Dental Pulp Cells And Its Effect On Osteoclastogenesis, Hataichanok Charoenpong
Induction Of S100a7 Expression From Mechanical Stress-Induced Human Dental Pulp Cells And Its Effect On Osteoclastogenesis, Hataichanok Charoenpong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Mechanical injuries of dental pulp tissues could lead to root resorption by osteoclast/odontoclast. S100A7 has been demonstrated to involve in inflammatory processes and potentially involved in bone destruction. However, the roles of S100A7 in dental pulp have not been reported. Therefore, this this study aimed to investigate the effect of mechanical stress on S100A7 expression in human dental pulp cells (hDPCs) and to investigate the effect of S100A7 protein on osteoclast differentiation. HDPCs were stimulated with in vitro compressive loading. Cell viability was determined using MTT assay. Real-time PCR (qPCR) and ELISA were utilized to determine the mRNA and protein …
Differential Dendritic Cell Responses To Candida Species, Thu Nguyen Ngoc
Differential Dendritic Cell Responses To Candida Species, Thu Nguyen Ngoc
Chulalongkorn University Theses and Dissertations (Chula ETD)
Mannan in the outermost layer of Candida cell wall may be the first molecules that interact with host dendritic cells (DCs) and activate immune responses that determine disease outcomes. However, little is known how different mannan structures of common Candida species affect DC activation and T cell responses. Thus, this work compared the effects of mannans of Candida albicans, Candida tropicalis, Candida glabrata, Candida krusei, Candida parapsilosis and Candida dubliniensis on bone marrow-derived DC (BMDC) responses in mice. C. albicans and C. tropicalis mannan could not activate DC maturation, however, they induced IFN-gamma production which consequently provoked Th1 responses. C. …