Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 3 of 3
Full-Text Articles in Medicine and Health Sciences
The Tetraspanin Cd82 Regulates Hematopoietic Stem Cell Fitness And Bone Marrow Retention, Chelsea Saito-Reis
The Tetraspanin Cd82 Regulates Hematopoietic Stem Cell Fitness And Bone Marrow Retention, Chelsea Saito-Reis
Biomedical Sciences ETDs
The maintenance of the hematopoietic stem and progenitor cell (HSPC) population is critical to sustaining the adult blood and immune system throughout an organism’s lifespan. The bone marrow microenvironment plays a key role in the regulation of HSPC maintenance and functions. Previous work from our lab has identified the tetraspanin CD82 as an important modulator of HSPC interactions with the bone marrow niche. However, the mechanisms as to how CD82 contributes to the maintenance, trafficking and retention of HSPCs with the niche remained unclear. First, we investigated how CD82 promotes HSPC quiescence, homing and engraftment using a global CD82 knock …
Cd82 Membrane Scaffolding Regulates Hematopoietic Cell Functions, Christina M. Termini
Cd82 Membrane Scaffolding Regulates Hematopoietic Cell Functions, Christina M. Termini
Biomedical Sciences ETDs
Through their ability to self-renew and differentiate, hematopoietic stem/progenitor cells (HSPCs) maintain the adult blood and immune systems. The microenvironment, or niche, in which HSPCs reside, serves as a critical regulator of HSPC functions. As previous work has identified the tetraspanin CD82 as a mediator of HSPC-niche interactions, we aimed to determine the mechanism by which this occurs. Our data demonstrate that CD82 expression and scaffolding regulate HSPC interactions with niche components by organizing the α4 integrin subunit into tightly packed nanoclusters. The HSPC niche can also protect acute myeloid leukemia (AML) cells from therapeutics. Therefore, we next examined how …
Cd82 Regulation Of Hematopoietic Stem/Progenitor Cell - Niche Adhesion, Maura L. Cotter
Cd82 Regulation Of Hematopoietic Stem/Progenitor Cell - Niche Adhesion, Maura L. Cotter
Biomedical Sciences ETDs
The spatial organization and dynamics of proteins and lipids within the cell membrane is important for the regulation of cell signaling, adhesion, and cell communication. Within the bone marrow niche, communication between hematopoietic stem/progenitor cells (HSPCs) and niche cells is essential for regulating their proliferation, differentiation, and survival. Our previous work has ascertained that HSPCs utilize a polarized domain on the plasma membrane that serves as the contact site with osteoblasts, which are important members of the bone marrow niche. Using human primary CD34+ stem/progenitor cells and the progenitor-like KG1a cell line, we found this domain to be enriched in …