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Dengue Virus Infection And Virus-Specific Hla-A2 Restricted Immune Responses In Humanized Nod-Scid Il2rgammanull Mice, Smita Jaiswal, Todd Pearson, Heather Friberg, Leonard D. Shultz, Dale L. Greiner, Alan L. Rothman, Anuja Mathew
Dengue Virus Infection And Virus-Specific Hla-A2 Restricted Immune Responses In Humanized Nod-Scid Il2rgammanull Mice, Smita Jaiswal, Todd Pearson, Heather Friberg, Leonard D. Shultz, Dale L. Greiner, Alan L. Rothman, Anuja Mathew
Alan Rothman
BACKGROUND: The lack of a suitable animal model to study viral and immunological mechanisms of human dengue disease has been a deterrent to dengue research.
METHODOLOGY/PRINCIPAL FINDINGS: We sought to establish an animal model for dengue virus (DENV) infection and immunity using non-obese diabetic/severe combined immunodeficiency interleukin-2 receptor gamma-chain knockout (NOD-scid IL2rgamma(null)) mice engrafted with human hematopoietic stem cells. Human CD45(+) cells in the bone marrow of engrafted mice were susceptible to in vitro infection using low passage clinical and established strains of DENV. Engrafted mice were infected with DENV type 2 by different routes and at multiple time points …