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Full-Text Articles in Medicine and Health Sciences
Altered Cytokine Responses Of Dengue-Specific Cd4+ T Cells To Heterologous Serotypes, Maloy Mangada, Alan Rothman
Altered Cytokine Responses Of Dengue-Specific Cd4+ T Cells To Heterologous Serotypes, Maloy Mangada, Alan Rothman
Alan Rothman
The interplay of different inflammatory cytokines induced during a dengue (DEN) virus infection plays a role in either protection or increased disease severity. We measured the frequencies and characterized the cytokine responses of DEN virus-specific memory CD4+ T cells in PBMC of six volunteers who received experimental live attenuated monovalent DEN vaccines. IFN-gamma and TNF-alpha responses to inactivated DEN Ags were detected in up to 0.54 and 1.17% of total circulating CD4+ T cells, respectively. Ags from the homologous serotype elicited the highest IFN-gamma response. The ratio of TNF-alpha- to IFN-gamma-producing CD4+ T cells was higher after stimulation with Ags …
Analysis Of Murine Cd8(+) T-Cell Clones Specific For The Dengue Virus Ns3 Protein: Flavivirus Cross-Reactivity And Influence Of Infecting Serotype, Anne C. Spaulding, Ichiro Kurane, Francis A. Ennis, Alan L. Rothman
Analysis Of Murine Cd8(+) T-Cell Clones Specific For The Dengue Virus Ns3 Protein: Flavivirus Cross-Reactivity And Influence Of Infecting Serotype, Anne C. Spaulding, Ichiro Kurane, Francis A. Ennis, Alan L. Rothman
Alan Rothman
Serotype-cross-reactive dengue virus-specific cytotoxic T lymphocytes (CTL) induced during a primary dengue virus infection are thought to play a role in the immunopathogenesis of dengue hemorrhagic fever (DHF) during a secondary dengue virus infection. Although there is no animal model of DHF, we previously reported that murine dengue virus-specific CTL responses are qualitatively similar to human dengue virus-specific CTL responses. We used BALB/c mice to study the specificity of the CTL response to an immunodominant epitope on the dengue virus NS3 protein. We mapped the minimal H-2Kd-restricted CTL epitope to residues 298 to 306 of the dengue type 2 virus …
Identification And Analysis For Cross-Reactivity Among Hantaviruses Of H-2b-Restricted Cytotoxic T-Lymphocyte Epitopes In Sin Nombre Virus Nucleocapsid Protein, Ken Maeda, Kim West, Tomoko Toyosaki-Maeda, Alan Rothman, Francis Ennis, Masanori Terajima
Identification And Analysis For Cross-Reactivity Among Hantaviruses Of H-2b-Restricted Cytotoxic T-Lymphocyte Epitopes In Sin Nombre Virus Nucleocapsid Protein, Ken Maeda, Kim West, Tomoko Toyosaki-Maeda, Alan Rothman, Francis Ennis, Masanori Terajima
Alan Rothman
Sin Nombre virus (SNV) causes hantavirus pulmonary syndrome (HPS), with a high rate of mortality in humans who are infected by the transmission of virus from the natural rodent host. In humans, cytotoxic T lymphocytes (CTL) specific for SNV appear to play an important role in the pathogenicity of HPS. There is a correlation between the frequencies of SNV-specific CTLs and the severity of HPS disease. In order to create a mouse model to study the role of SNV-specific T cells in vivo, T cell responses to SNV nucleocapsid (N) protein in B6.PL Thy1(a)/Cy mice (H-2(b)) immunized with plasmid DNA …
Identification Of Murine Poxvirus-Specific Cd8+ Ctl Epitopes With Distinct Functional Profiles, Anuja Mathew, Masanori Terajima, Kim West, Sharone Green, Alan Rothman, Francis Ennis, Jeffrey Kennedy
Identification Of Murine Poxvirus-Specific Cd8+ Ctl Epitopes With Distinct Functional Profiles, Anuja Mathew, Masanori Terajima, Kim West, Sharone Green, Alan Rothman, Francis Ennis, Jeffrey Kennedy
Alan Rothman
Murine T cell epitopes against vaccinia virus (VV) have not been characterized to date in part due to the large and complex genome of VV. We have identified and characterized two CD8+ T cell epitopes on the A47L (modified VV Ankara strain (MVA)-029) and J6R (MVA-043) proteins of VV that are Db and Kb restricted, respectively. Following i.p. immunization with VV New York City Board of Health (NYCBH) strain, MVA-029 peptide-stimulated splenocytes secreted IFN-gamma from 7 days to 7 mo postimmunization, and virus-stimulated effectors were also able to lyse MVA-029-pulsed target cells at the same time points. In contrast, MVA-043 …