Open Access. Powered by Scholars. Published by Universities.®
Zucker School of Medicine at Hofstra/Northwell
Milk fat globule-epidermal growth factor-factor 8;; neutrophil;; HL-60;; cells;; MAP kinase;; p38;; ERK;; G protein-coupled receptor kinase 2;; CXCR2;; acute lung injury;; developmental endothelial locus-1;; respiratory-distress-syndrome;; ischemia-reperfusion injury;; coupled;; receptor kinases;; discoidin-domain protein;; egf factor-viii;; growth-factor;; apoptotic cells;; melanoma progression;; Research & Experimental Medicine
Articles 1 - 1 of 1
Full-Text Articles in Medicine and Health Sciences
Mfg-E8 Inhibits Neutrophil Migration Through Alpha(V)Beta(3)-Integrin-Dependent Map Kinase Activation, M. Aziz, W. L. Yang, L. M. Corbo, W. W. Chaung, S. Matsuo, P. Wang
Mfg-E8 Inhibits Neutrophil Migration Through Alpha(V)Beta(3)-Integrin-Dependent Map Kinase Activation, M. Aziz, W. L. Yang, L. M. Corbo, W. W. Chaung, S. Matsuo, P. Wang
Journal Articles
We have previously demonstrated the involvement of milk fat globule-epidermal growth factor-factor 8 (MFG-E8) in reducing neutrophil infiltration in a murine model of acute lung injury (ALI). In the present study, we aimed to delineate the mechanisms through which MFG-E8 attenuates neutrophil migration. Recombinant human MFG-E8 (rhMFG-E8) was expressed and purified in our facility. The human differentiated neutrophil cell line, dHL-60, was treated with rhMFG-E8 and cell migration assay was performed in a Boyden chamber using recombinant interleukin-8 (IL-8) as the chemoattractant. Surface CXCR2 and intracellular G protein-coupled receptor kinase 2 (GRK2) levels were evaluated by flow cytometry or western …