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Full-Text Articles in Medicine and Health Sciences
Preparation And Using Phantom Lesions To Practice Fine Needle Aspiration Biopsies, Vinod B. Shidham, George M. Varsegi, Krista D’Amore, Anjani Shidham
Preparation And Using Phantom Lesions To Practice Fine Needle Aspiration Biopsies, Vinod B. Shidham, George M. Varsegi, Krista D’Amore, Anjani Shidham
Department of Pathology
Currently, health workers including residents and fellows do not have a suitable phantom model to practice the fine- needle aspiration biopsy (FNAB) procedure. In the past, we standardized a model consisting of latex glove containing fresh cattle liver for practicing FNAB. However, this model is difficult to organize and prepare on short notice, with the procurement of fresh cattle liver being the most challenging aspect. Handling of liver with contamination-related problems is also a significant draw back. In addition, the glove material leaks after a few needle passes, with resulting mess.
We have established a novel simple method of embedding …
Cell Block Preparation From Cytology Specimen With Predominance Of Individually Scattered Cells, George M. Varsegi, Vinod B. Shidham
Cell Block Preparation From Cytology Specimen With Predominance Of Individually Scattered Cells, George M. Varsegi, Vinod B. Shidham
Department of Pathology
This article with accompanied video demonstrates Shidham's method for preparation of cell blocks from liquid based cervicovaginal cytology specimens containing individually scattered cells and small cell groups. This technique uses HistoGel (Thermo Scientific) with conventional laboratory equipment.
The use of cell block sections is a valuable ancillary tool for evaluation of non-gynecologic cytology. They enable the cytopathologist to study additional morphologic specimen detail including the architecture of the lesion. Most importantly, they allow for the evaluation of ancillary studies such as immunocytochemistry, in-situ hybridization tests (FISH/CISH) and in-situ polymerase chain reaction (PCR). Traditional cell block preparation techniques have mostly been …