Medicine and Health Sciences Commons^™

B1 Cell Ige Impedes Mast Cell-Mediated Enhancement Of Parasite Expulsion Through B2 Ige Blockade, Rebecca K. Martin, Sheela R. Damle, Yolander A. Valentine, Matthew P. Zellner, Briana N. James, Joseph C. Lownik, Andrea J. Luker, Elijah H. Davis, Martha M. Demeules, Laura M. Khandjian, Fred D. Finkelman, Joseph F. Urban Jr., Daniel H. Conrad

Microbiology and Immunology Publications

Helminth infection is known for generating large amounts of poly-specific IgE. Here we demonstrate that innate-like B1 cells are responsible for this IgE production during infection with the nematode parasites Nippostrongylus brasiliensis and Heligmosomoides polygyrus bakeri. In vitro analysis of B1 cell immunoglobulin class switch recombination to IgE demonstrated a requirement for anti-CD40 and IL-4 that was further enhanced when IL-5 was added or when the B1 source was helminth infected mice. An IL-25-induced upregulation of IgE in B1 cells was also demonstrated. In T cell-reconstituted RAG1^−/− mice, N. brasiliensis clearance was enhanced with the addition of B2 …